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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-52608 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Carbonic Anhydrase XII Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: TASNKSEGLA VLAVLIEMGS FNPSYDKIFS HLQHVKYKGQ EAFVPGFNIE ELLPERTAEY YRYRGSLTTP PCNPTVLWTV FRNPVQISQE QLLALETALY CTHMDDPSPR EMINNFRQVQ Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 88%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.08 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Carbonic Anhydrase Inhibitor Acetazolamide Enhances CHOP Treatment Response and Stimulates Effector T-Cell Infiltration in A20/BalbC Murine B-Cell Lymphoma.
Méhes G, Matolay O, Beke L, Czenke M, Pórszász R, Mikó E, Bai P, Berényi E, Trencsényi G
International journal of molecular sciences 2020 Jul 15;21(14)
International journal of molecular sciences 2020 Jul 15;21(14)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Carbonic Anhydrase XII in human cell line RT4 using a Carbonic Anhydrase XII Polyclonal Antibody (Product # PA5-52608) shows localization to nucleus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 CAIX and CAXII presented by immunohistochemistry and Western blotting of A20 murine lymphoma grafts. Uniform weak cytoplasmic/membrane CAIX positivity of lymphoma cells ( A ); heterogeous IHC reaction with slight increase in the giant cell component following CHOP treatment, note negativity for the immune infiltrate ( B ); negative IHC staining for CAXII ( C ) (40x magnification). Western blot of A20 lymphoma cell lysate presenting a single specific band at 53-55 kDa for CAIX (center lane) and no signal for the CAXII protein ( D , right lane, arrow). No unspecific signal by the detection system (based on anti-igG-HRP) applied in the absence of the primary antibodies was seen (negative control, left lane).
