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antibody from Invitrogen Antibodies
Targeting: MISP
C19orf21, Caprice, DKFZp686H18209, MISP1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [6]
- Other assay [1]
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- Product number
- PA5-61995 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C19orf21 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SQASGITGSY SVSESPFFSP IHLHSNVAWT VEDPVDSAPP GQRKKEQWYA GINPSDGINS EVLEAIRVTR HKNAMAERWE SRIYASEEDD
- Concentration
- 0.2 mg/mL
Submitted references Mitotic Spindle Positioning (MISP) is an actin bundler that selectively stabilizes the rootlets of epithelial microvilli.
Morales EA, Arnaiz C, Krystofiak ES, Zanic M, Tyska MJ
Cell reports 2022 Apr 19;39(3):110692
Cell reports 2022 Apr 19;39(3):110692
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C19orf21 in control (vector only transfected HEK293T lysate) and MISP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a C19orf21 Polyclonal Antibody (Product # PA5-61995).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of C19orf21 in human cell line U-2 OS shows positivity in plasma membrane & focal adhesion sites. Samples were probed using a C19orf21 Polyclonal Antibody (Product # PA5-61995).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of C19orf21 in human cell line U-2 OS using a C19orf21 Polyclonal Antibody (Product # PA5-61995) shows localization to plasma membrane and focal adhesion sites.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of C19orf21 in human testis using C19orf21 Polyclonal Antibody (Product # PA5-61995).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of C19orf21 in human kidney using C19orf21 Polyclonal Antibody (Product # PA5-61995).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of C19orf21 in human small intestine shows strong membranous positivity in glandular cells. Samples were probed using a C19orf21 Polyclonal Antibody (Product # PA5-61995).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of C19orf21 in human small intestine and liver tissues using C19orf21 Polyclonal Antibody (Product # PA5-61995). Corresponding MISP RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of C19orf21 in human kidney, liver, small intestine and testis using C19orf21 Polyclonal Antibody (Product # PA5-61995) (A) shows similar protein distribution across tissues to an independent C19orf21 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of C19orf21 in human liver using C19orf21 Polyclonal Antibody (Product # PA5-61995) shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. MISP localizes to microvillar rootlets (A) Confocal images of small intestinal sections stained for MISP (green), villin (magenta), DNA with DRAQ5 (yellow), and membrane with WGA (cyan). Panel shows a split three-color merge. Scale bar: 15 mum. (B) Fluorescence intensity distributions of MISP, villin, and apical membrane measured parallel to the microvillar axis as indicated by the white arrow in (A); n = 200 line scans measured on five villi. (C) Inverted MISP and villin channels from (A). Scale bar: 15 mum. (D) Fluorescence intensity distribution of MISP and villin measured relative to the crypt-villus axis as indicated by the highlighted area in (C); black arrow shows line scan orientation; n = 11 scans of six villi. (E) Confocal maximum intensity projection (Max IP) of CACO-2 BBE cells at different stages of differentiation stained for MISP (green) and F-actin with phalloidin (magenta). Upper panels show xy en face views at the indicated days post-confluency (DPC); bottom panels show resliced xz views. Scale bar: 3 mum. (F) Western blot time series of CACO-2 BBE cell lysates probed for MISP, villin, beta-actin, and GAPDH at the indicated DPC. (G) Density values of MISP, villin, and beta-actin bands from F normalized to GAPDH and plotted as a function of DPC. (H) SIM Max IP of a W4 cell stained for MISP (green), F-actin with phalloidin (magenta), and membrane with WGA (cyan). Panel shows a split two-color merge. Scale bar: 3 mum. (I) Fluorescence intensity distri
