NBP2-14390

antibody from Novus Biologicals

Targeting: MISP C19orf21, Caprice, DKFZp686H18209, MISP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-14390

Provider

Novus Biologicals

Product name

Rabbit Polyclonal MISP1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human C19orf21 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MISP1 Antibody [NBP2-14390] - Analysis in control (vector only transfected HEK293T lysate) and MISP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390] - Staining of human stomach, lower shows strong membranous and cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390] - Staining of human liver shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390] - Staining of human small intestine shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390] - Staining of human kidney.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390] - Analysis in human small intestine and liver tissues. Corresponding MISP1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390] - Staining of human kidney, liver, small intestine and testis using Anti-MISP1 antibody NBP2-14390 (A) shows similar protein distribution across tissues to independent antibody NBP2-38955 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390] - Staining of human kidney shows moderate positivity in luminal membrane in cells in tubules.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390] - Staining of human liver shows no positivity in hepatocytes as expected.