Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-20728 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TNFAIP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is 293 cell lysate.
- Concentration
- 1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SK-N-SH cell lysate using a TNFAIP3 polyclonal antibody (Product # PA5-20728) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TNFAIP3 in SK-N-SH cell lysate with TNFAIP3 Polyclonal Antibody (Product # PA5-20728) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-TNFAIP3 Polyclonal Antibody (Product # PA5-20728) and a 80kDa band corresponding to TNFAIP3 was observed in all the tested cell models and increased upon using different treatments. Whole cell lysates (30ug lysate) of RAW 264.7 (Lane 1), RAW 264.7 treated with Lipopolysaccharide (1µg/ml, 12 hours) (Lane 2), RAW 264.7 treated with TNFa (20ng/mL, 12 hours) (Lane 3) and HeLa (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1µg/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of human lung cells using a TNFAIP3 polyclonal antibody (Product # PA5-20728) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of TNFAIP3 in Human Lung cells with TNFAIP3 Polyclonal Antibody (Product # PA5-20728) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of TNFAIP3 in human lung tissue with TNFAIP3 Polyclonal Antibody (Product # PA5-20728) at 5 µg/mL.