Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA324651 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal PI3KC3 Antibody (S34)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal PI3KC3 Antibody (S34)
- Reactivity
- Porcine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PIK3C3
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of PI3KC3 (S34) (Cat. #TA324651) in Hela cell line lysates (35ug/lane). PI3KC3 (arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of U251 cells stained with PI3KC3 (S34) antibody. U251 cells were treated with Chloroquine , then incubated with TA324651 PI3KC3 (S34) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . PI3KC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PI3KC3 Antibody (S34), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
