PAB28435

antibody from Abnova Corporation

Targeting: ASCC2 ASC1p100, DKFZp586O0223, FLJ21588

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28435

Provider

Abnova Corporation

Product name

ASCC2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ASCC2.

Antigen sequence

YSVVVEEVPLQPGESLPYHSVYYEDEYDDTYDGNQ
VGANDADSDDELISRRPFTIPQVLRTKVPREGQEE
DDDDEEDDADEEAPKPDHFVQDPAVLREKAEARRM
AFLAKKGYRHDSSTAVAGSPRGHGQSRETTQERRK
KEANKATRANH


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: EFO-21, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with ASCC2 polyclonal antibody (Cat # PAB28435) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of U-2 OS with ASCC2 polyclonal antibody (Cat # PAB28435) at 1-4 ug/mL dilution shows positivity in nucleus, cytoplasm & focal adhesions.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human duodenum with ASCC2 polyclonal antibody (Cat # PAB28435) shows strong cytoplasmic positivity in glandular cells at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)