Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- BS61336 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- SRD5A1 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to residues in Human SRD5A1.
- Description
- Steroid 5Ï«-Reductase is an important enzyme in androgen physiology because it catalyzes the conversion of testosterone into the more potent 5Ï«-dihydro-testosterone, which mediates androgen effects on target tissues. The enzyme exists as two isoforms: type 1, which is expressed mainly in the skin; and type 2, which is expressed mainly in the prostate. In cultured human skin cells, 5Ï«-Reductase 1 shows heterogeneity of protein, and has different levels of transcriptional and translational expression. 5Ï«-Reductase 1 is expressed in all portions of the hair follicle, whereas 5Ï«-Reductase 2 is expressed only in mesenchymal portions. In addition, 5Ï«-Reductase 1 is mainly expressed in human breast carcinoma and may play a role in the in situ production and actions of the potent androgen 5Ï«-dihydrotestosterone, including inhibition of cancer cell proliferation in hormone-dependent human breast carcinoma. The 5Ï«-Reductase-3Ï«-hydroxysteroid dehydrogenase complex is present in the human brain, suggesting that the complex may be involved in the synthesis of neuroactive steroids or the catabolism of neurotoxic steroids.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of SRD5A1 pAb at 1:500 dilutionLine1:HEK293T whole cell lysateLine2:H9C2 whole cell lysateLine3:Raw264.7 whole cell lysate