66329-1-IG

antibody from Invitrogen Antibodies

Targeting: SRD5A1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

66329-1-IG

Provider

Invitrogen Antibodies

Product name

SRD5A1 Monoclonal Antibody (1D5D8)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: MATATGVAE ERLLAALAYL QCAVGCAVFA RNRQTNSVYG RHALPSHRLR VPARAAWVVQ ELPSLALPLY QYASESAPRL RSAPNCILLA MFLVHYGHRC LIYPFLMRGG KPMPLLACTM AIMFCTCNGY LQSRYLSHCA VYADDWVTDP RFLIGFGLWL TGML (1-163 aa encoded by BC007033)

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1D5D8

Vial size

150 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human testis tissue were subjected to SDS PAGE followed by western blot with 66329-1-IG ( SRD5A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 66329-1-IG (SRD5A1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 66329-1-IG (SRD5A1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 66329-1-IG ( SRD5A1 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 66329-1-IG ( SRD5A1 Antibody) at dilution of 1:200 (under 40x lens).