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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- UM500058 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM500058, RRID:AB_2629071
- Product name
- SLC7A8 mouse monoclonal antibody,clone UMAB70
- Antibody type
- Monoclonal
- Description
- SLC7A8 mouse monoclonal antibody,clone UMAB70
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SLC7A8
- Isotype
- IgG
- Antibody clone number
- UMAB70
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid 9: Colon, 10: Spleen). Diluation: 1:500.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-SLC7A8 mouse monoclonal antibody (UM500058, green, 1:50). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human normal colon tissue and colorectal cancer tissue using anti-SLC7A8 mouse monoclonal antibody. (UM500058; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human normal kidney tissue and kidney carcinoma tissue using anti-SLC7A8 mouse monoclonal antibody. (UM500058; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human normal pancreas tissue and pancreatic cancer tissue using anti-SLC7A8 mouse monoclonal antibody. (UM500058; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-SLC7A8 mouse monoclonal antibody (UM500058). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
- Validation comment
- 10K-CHIP
