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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA500503 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500503, RRID:AB_2286226
- Product name
- Anti-SLC7A8 (LAT2) mouse monoclonal antibody, clone OTI5A9 (formerly 5A9)
- Antibody type
- Monoclonal
- Description
- Anti-SLC7A8 (LAT2) mouse monoclonal antibody, clone OTI5A9 (formerly 5A9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SLC7A8
- Isotype
- IgG
- Antibody clone number
- OTI5A9
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Expression of large neutral amino acid transporters LAT1 and LAT2 in medulloblastoma.
Cicone F, Minniti G, Oliva MA, Carideo L, Prior JO, Scopinaro F, Giangaspero F, Arcella A
Brain tumor pathology 2017 Oct;34(4):179-181
Brain tumor pathology 2017 Oct;34(4):179-181
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC7A8 (RC208586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC7A8.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SLC7A8 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SLC7A8 mouse monoclonal antibody (TA500503) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SLC7A8(RC208586).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of SLC7A8 by using TrueMab monoclonal anti-SLC7A8 antibodies (Negative control: IP without adding anti-SLC7A8 antibody.). For each experiment, 500ul of DDK tagged SLC7A8 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SLC7A8 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SLC7A8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500503)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC208586 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SLC7A8 antibody(TA500503), and then analyzed by flow cytometry.
- Validation comment
- FC
