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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA500513 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500513, RRID:AB_2239464
- Product name
- Anti-SLC7A8 (LAT2) mouse monoclonal antibody, clone OTI8B12 (formerly 8B12)
- Antibody type
- Monoclonal
- Description
- Anti-SLC7A8 (LAT2) mouse monoclonal antibody, clone OTI8B12 (formerly 8B12)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SLC7A8
- Isotype
- IgG
- Antibody clone number
- OTI8B12
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references Methylmercury Uptake into BeWo Cells Depends on LAT2-4F2hc, a System L Amino Acid Transporter.
Balthasar C, Stangl H, Widhalm R, Granitzer S, Hengstschläger M, Gundacker C
International journal of molecular sciences 2017 Aug 8;18(8)
International journal of molecular sciences 2017 Aug 8;18(8)
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC7A8 (RC208586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC7A8.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-SLC7A8 monoclonal antibody at 1:200 dilution.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-SLC7A8mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500513, Dilution 1:50)
- Validation comment
- IHC
