TA500514

antibody from OriGene

Targeting: SLC7A8 LAT2, LPI-PC1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500514

Provider

OriGene

Proper citation

OriGene Cat#TA500514, RRID:AB_11124222

Product name

SLC7A8 (LAT2) mouse monoclonal antibody, clone OTI4A6 (formerly 4A6)

Antibody type

Monoclonal

Description

SLC7A8 (LAT2) mouse monoclonal antibody, clone OTI4A6 (formerly 4A6)

Host

Mouse

Conjugate

Unconjugated

Epitope

SLC7A8

Isotype

IgG

Antibody clone number

OTI4A6

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SLC7A8 monoclonal antibody.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC7A8 (RC208586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC7A8.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-SLC7A8 mouse monoclonal antibody (TA500514) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SLC7A8(RC208586).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC208586 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SLC7A8 antibody(TA500514), and then analyzed by flow cytometry.

Validation comment

FC