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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA500632 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500632, RRID:AB_11126656
- Product name
- Anti-SLC7A8 (LAT2) mouse monoclonal antibody, clone OTI4H10 (formerly 4H10)
- Antibody type
- Monoclonal
- Description
- Anti-SLC7A8 (LAT2) mouse monoclonal antibody, clone OTI4H10 (formerly 4H10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SLC7A8
- Isotype
- IgG
- Antibody clone number
- OTI4H10
- Vial size
- 100 µl
- Concentration
- 0.65 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SLC7A8 mouse monoclonal antibody (TA500632) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SLC7A8(RC208586).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-SLC7A8 mouse monoclonal antibody (TA500632).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of SLC7A8 by using TrueMab monoclonal anti-SLC7A8 antibodies (Negative control: IP without adding anti-SLC7A8 antibody.). For each experiment, 500ul of DDK tagged SLC7A8 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SLC7A8 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-SLC7A8 antibody(TA500632),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
