Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- HPA036947 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA036947, RRID:AB_10671367
- Product name
- Anti-NCAPD2
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human NCAPD2, Gene description: non-SMC condensin I complex, subunit D2, Alternative Gene Names: CAP-D2, CNAP1, hCAP-D2, KIAA0159, Validated applications: WB, IHC, Uniprot ID: Q15021, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Cell division requires RNA eviction from condensing chromosomes
Live imaging of marked chromosome regions reveals their dynamic resolution and compaction in mitosis
Sharp J, Perea-Resa C, Wang W, Blower M
Journal of Cell Biology 2020;219(11)
Journal of Cell Biology 2020;219(11)
Live imaging of marked chromosome regions reveals their dynamic resolution and compaction in mitosis
Eykelenboom J, GierliĆski M, Yue Z, Hegarat N, Pollard H, Fukagawa T, Hochegger H, Tanaka T
Journal of Cell Biology 2019;218(5):1531-1552
Journal of Cell Biology 2019;218(5):1531-1552
No comments: Submit comment
Enhanced validation
- Submitted by
- klas2
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting NCAPD2 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting NCAPD2 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
- Sample type
- U-2 OS
- Primary Ab dilution
- 1:58
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:3000
- Knockdown/Genetic Approaches Application
- Western blot