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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-58851 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOP14 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: REKPKSRKEL IEELIAKSKQ EKRERQAQRE DALELTEKLD QDWKEIQTLL SHKTPKSENR DKKEKPKPDA YDMMVRELGF EMKAQPSNRM KT Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 89%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NOP14 in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a NOP14 Polyclonal Antibody (Product # PA5-58851). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NOP14 in mouse cell line NIH-3T3 and rat cell line NBT-II using a NOP14 Polyclonal Antibody (Product # PA5-58851).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NOP14 in human cell line U-2 OS shows positivity in nucleus & nucleoli. Samples were probed using a NOP14 Polyclonal Antibody (Product # PA5-58851).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of NOP14 in human cell line U-2 OS using NOP14 Polyclonal Antibody (Product # PA5-58851). Staining shows localization to nucleus and nucleoli.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NOP14 in human hippocampus using NOP14 Polyclonal Antibody (Product # PA5-58851) shows moderate nucleolar positivity in neurons.
