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- References [22]
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- Product number
- 66137-1-Ig - Provider product page
- Provider
- Proteintech Group
- Product name
- IFITM2-specific antibody
- Antibody type
- Monoclonal
- Description
- KD/KO validated IFITM2-specific antibody (Cat. #66137-1-Ig) is a mouse monoclonal antibody that shows reactivity with human and has been validated for the following applications: FC, WB,ELISA.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 3D5F7
- Vial size
- 20ul, 150ul
Submitted references Caveats of Using Overexpression Approaches to Screen Cellular Host IFITM Proteins for Antiviral Activity.
Comparison of a Genotype 1 and a Genotype 2 Macaque Foamy Virus env Gene Indicates Distinct Infectivity and Cell-Cell Fusion but Similar Tropism and Restriction of Cell Entry by Interferon-Induced Transmembrane Proteins.
HIV-1 Nef Changes the Proteome of T Cells Extracellular Vesicles Depleting IFITMs and Other Antiviral Factors.
IFITM3 blocks influenza virus entry by sorting lipids and stabilizing hemifusion.
Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular model.
The Role of IFITM Proteins in Tick-Borne Encephalitis Virus Infection.
Emergent Role of IFITM1/3 towards Splicing Factor (SRSF1) and Antigen-Presenting Molecule (HLA-B) in Cervical Cancer.
Rapalogs downmodulate intrinsic immunity and promote cell entry of SARS-CoV-2.
IFITM Proteins That Restrict the Early Stages of Respiratory Virus Infection Do Not Influence Late-Stage Replication.
Long Noncoding RNA IFITM4P Regulates Host Antiviral Responses by Acting as a Competing Endogenous RNA.
Human IFITM3 restricts chikungunya virus and Mayaro virus infection and is susceptible to virus-mediated counteraction.
Interferon-Induced Transmembrane Proteins Inhibit Infection by the Kaposi's Sarcoma-Associated Herpesvirus and the Related Rhesus Monkey Rhadinovirus in a Cell-Specific Manner.
p53 promotes ZDHHC1-mediated IFITM3 palmitoylation to inhibit Japanese encephalitis virus replication.
The Robust Restriction of Zika Virus by Type-I Interferon in A549 Cells Varies by Viral Lineage and Is Not Determined by IFITM3.
IFITM3 directly engages and shuttles incoming virus particles to lysosomes.
IFITM proteins inhibit HIV-1 protein synthesis.
mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3.
A CRISPR screen for factors regulating SAMHD1 degradation identifies IFITMs as potent inhibitors of lentiviral particle delivery.
Intrinsic Immunity Shapes Viral Resistance of Stem Cells.
Zika virus induces massive cytoplasmic vacuolization and paraptosis-like death in infected cells.
Δ20 IFITM2 differentially restricts X4 and R5 HIV-1.
IFITM3-containing exosome as a novel mediator for anti-viral response in dengue virus infection.
Meischel T, Fritzlar S, Villalón-Letelier F, Smith JM, Brooks AG, Reading PC, Londrigan SL
Pathogens (Basel, Switzerland) 2023 Mar 27;12(4)
Pathogens (Basel, Switzerland) 2023 Mar 27;12(4)
Comparison of a Genotype 1 and a Genotype 2 Macaque Foamy Virus env Gene Indicates Distinct Infectivity and Cell-Cell Fusion but Similar Tropism and Restriction of Cell Entry by Interferon-Induced Transmembrane Proteins.
Fricke T, Schlagowski S, Liu S, Yang X, Fiebig U, Kaul A, Ensser A, Hahn AS
Viruses 2023 Jan 17;15(2)
Viruses 2023 Jan 17;15(2)
HIV-1 Nef Changes the Proteome of T Cells Extracellular Vesicles Depleting IFITMs and Other Antiviral Factors.
da Silva-Januário ME, da Costa CS, Tavares LA, Oliveira AK, Januário YC, de Carvalho AN, Cassiano MHA, Rodrigues RL, Miller ME, Palameta S, Arns CW, Arruda E, Paes Leme AF, daSilva LLP
Molecular & cellular proteomics : MCP 2023 Dec;22(12):100676
Molecular & cellular proteomics : MCP 2023 Dec;22(12):100676
IFITM3 blocks influenza virus entry by sorting lipids and stabilizing hemifusion.
Klein S, Golani G, Lolicato F, Lahr C, Beyer D, Herrmann A, Wachsmuth-Melm M, Reddmann N, Brecht R, Hosseinzadeh M, Kolovou A, Makroczyova J, Peterl S, Schorb M, Schwab Y, Brügger B, Nickel W, Schwarz US, Chlanda P
Cell host & microbe 2023 Apr 12;31(4):616-633.e20
Cell host & microbe 2023 Apr 12;31(4):616-633.e20
Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular model.
Cheng Y, Medina A, Yao Z, Basu M, Natekar JP, Lang J, Sanchez E, Nkembo MB, Xu C, Qian X, Nguyen PTT, Wen Z, Song H, Ming GL, Kumar M, Brinton MA, Li MMH, Tang H
Cell reports 2022 May 31;39(9):110885
Cell reports 2022 May 31;39(9):110885
The Role of IFITM Proteins in Tick-Borne Encephalitis Virus Infection.
Chmielewska AM, Gómez-Herranz M, Gach P, Nekulova M, Bagnucka MA, Lipińska AD, Rychłowski M, Hoffmann W, Król E, Vojtesek B, Sloan RD, Bieńkowska-Szewczyk K, Hupp T, Ball K
Journal of virology 2022 Jan 12;96(1):e0113021
Journal of virology 2022 Jan 12;96(1):e0113021
Emergent Role of IFITM1/3 towards Splicing Factor (SRSF1) and Antigen-Presenting Molecule (HLA-B) in Cervical Cancer.
Gómez-Herranz M, Faktor J, Yébenes Mayordomo M, Pilch M, Nekulova M, Hernychova L, Ball KL, Vojtesek B, Hupp TR, Kote S
Biomolecules 2022 Aug 8;12(8)
Biomolecules 2022 Aug 8;12(8)
Rapalogs downmodulate intrinsic immunity and promote cell entry of SARS-CoV-2.
Shi G, Chiramel AI, Li T, Lai KK, Kenney AD, Zani A, Eddy A, Majdoul S, Zhang L, Dempsey T, Beare PA, Kar S, Yewdell JW, Best SM, Yount JS, Compton AA
bioRxiv : the preprint server for biology 2022 Aug 30;
bioRxiv : the preprint server for biology 2022 Aug 30;
IFITM Proteins That Restrict the Early Stages of Respiratory Virus Infection Do Not Influence Late-Stage Replication.
Meischel T, Fritzlar S, Villalon-Letelier F, Tessema MB, Brooks AG, Reading PC, Londrigan SL
Journal of virology 2021 Sep 27;95(20):e0083721
Journal of virology 2021 Sep 27;95(20):e0083721
Long Noncoding RNA IFITM4P Regulates Host Antiviral Responses by Acting as a Competing Endogenous RNA.
Xiao M, Chen Y, Wang S, Liu S, Rai KR, Chen B, Li F, Li Y, Maarouf M, Chen JL
Journal of virology 2021 Oct 13;95(21):e0027721
Journal of virology 2021 Oct 13;95(21):e0027721
Human IFITM3 restricts chikungunya virus and Mayaro virus infection and is susceptible to virus-mediated counteraction.
Franz S, Pott F, Zillinger T, Schüler C, Dapa S, Fischer C, Passos V, Stenzel S, Chen F, Döhner K, Hartmann G, Sodeik B, Pessler F, Simmons G, Drexler JF, Goffinet C
Life science alliance 2021 Jul;4(7)
Life science alliance 2021 Jul;4(7)
Interferon-Induced Transmembrane Proteins Inhibit Infection by the Kaposi's Sarcoma-Associated Herpesvirus and the Related Rhesus Monkey Rhadinovirus in a Cell-Specific Manner.
Hörnich BF, Großkopf AK, Dcosta CJ, Schlagowski S, Hahn AS
mBio 2021 Dec 21;12(6):e0211321
mBio 2021 Dec 21;12(6):e0211321
p53 promotes ZDHHC1-mediated IFITM3 palmitoylation to inhibit Japanese encephalitis virus replication.
Wang X, Wu Z, Li Y, Yang Y, Xiao C, Liu X, Xiang X, Wei J, Shao D, Liu K, Deng X, Wu J, Qiu Y, Li B, Ma Z
PLoS pathogens 2020 Oct;16(10):e1009035
PLoS pathogens 2020 Oct;16(10):e1009035
The Robust Restriction of Zika Virus by Type-I Interferon in A549 Cells Varies by Viral Lineage and Is Not Determined by IFITM3.
Gobillot TA, Humes D, Sharma A, Kikawa C, Overbaugh J
Viruses 2020 May 2;12(5)
Viruses 2020 May 2;12(5)
IFITM3 directly engages and shuttles incoming virus particles to lysosomes.
Spence JS, He R, Hoffmann HH, Das T, Thinon E, Rice CM, Peng T, Chandran K, Hang HC
Nature chemical biology 2019 Mar;15(3):259-268
Nature chemical biology 2019 Mar;15(3):259-268
IFITM proteins inhibit HIV-1 protein synthesis.
Lee WJ, Fu RM, Liang C, Sloan RD
Scientific reports 2018 Sep 28;8(1):14551
Scientific reports 2018 Sep 28;8(1):14551
mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3.
Shi G, Ozog S, Torbett BE, Compton AA
Proceedings of the National Academy of Sciences of the United States of America 2018 Oct 23;115(43):E10069-E10078
Proceedings of the National Academy of Sciences of the United States of America 2018 Oct 23;115(43):E10069-E10078
A CRISPR screen for factors regulating SAMHD1 degradation identifies IFITMs as potent inhibitors of lentiviral particle delivery.
Roesch F, OhAinle M, Emerman M
Retrovirology 2018 Mar 20;15(1):26
Retrovirology 2018 Mar 20;15(1):26
Intrinsic Immunity Shapes Viral Resistance of Stem Cells.
Wu X, Dao Thi VL, Huang Y, Billerbeck E, Saha D, Hoffmann HH, Wang Y, Silva LAV, Sarbanes S, Sun T, Andrus L, Yu Y, Quirk C, Li M, MacDonald MR, Schneider WM, An X, Rosenberg BR, Rice CM
Cell 2018 Jan 25;172(3):423-438.e25
Cell 2018 Jan 25;172(3):423-438.e25
Zika virus induces massive cytoplasmic vacuolization and paraptosis-like death in infected cells.
Monel B, Compton AA, Bruel T, Amraoui S, Burlaud-Gaillard J, Roy N, Guivel-Benhassine F, Porrot F, Génin P, Meertens L, Sinigaglia L, Jouvenet N, Weil R, Casartelli N, Demangel C, Simon-Lorière E, Moris A, Roingeard P, Amara A, Schwartz O
The EMBO journal 2017 Jun 14;36(12):1653-1668
The EMBO journal 2017 Jun 14;36(12):1653-1668
Δ20 IFITM2 differentially restricts X4 and R5 HIV-1.
Wu WL, Grotefend CR, Tsai MT, Wang YL, Radic V, Eoh H, Huang IC
Proceedings of the National Academy of Sciences of the United States of America 2017 Jul 3;114(27):7112-7117
Proceedings of the National Academy of Sciences of the United States of America 2017 Jul 3;114(27):7112-7117
IFITM3-containing exosome as a novel mediator for anti-viral response in dengue virus infection.
Zhu X, He Z, Yuan J, Wen W, Huang X, Hu Y, Lin C, Pan J, Li R, Deng H, Liao S, Zhou R, Wu J, Li J, Li M
Cellular microbiology 2015 Jan;17(1):105-18
Cellular microbiology 2015 Jan;17(1):105-18
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Supportive validation
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The IFITM2-specific antibody from Proteintech is a mouse monoclonal antibody to a fusion protein of human IFITM2-specific. This antibody recognizes human antigen. The IFITM2-specific antibody has been validated for the following applications: ELISA, WB analysis.
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IFITM1 (Lane 1), IFITM2 (Lane 2) and IFITM3 (Lane 3) fusion proteins (20ng/lane) were subjected to SDS-PAGE followed by western blot with IFITM2 monoclonal antibody (66137-1-Ig) at dilution of 1:10,000.
- Sample type
- tissue
