Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-15778 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal MCAM/CD146 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references ImmunoPET Imaging of CD146 in Murine Models of Intrapulmonary Metastasis of Non-Small Cell Lung Cancer.
England CG, Jiang D, Hernandez R, Sun H, Valdovinos HF, Ehlerding EB, Engle JW, Yang Y, Huang P, Cai W
Molecular pharmaceutics 2017 Oct 2;14(10):3239-3247
Molecular pharmaceutics 2017 Oct 2;14(10):3239-3247
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCAM/CD146 Antibody [NBP2-15778] - A. 50 ug rat liver extract 7.5 % SDS-PAGE CD146 antibody [C1C3] dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCAM/CD146 Antibody [NBP2-15778] - A. 30 ug HUVEC whole cell lysate/extract 7.5 % SDS-PAGE CD146 antibody [C1C3] dilution: 1:10000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCAM/CD146 Antibody [NBP2-15778] - Wild-type (WT) and CD146 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCAM/CD146 Antibody [NBP2-15778] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778] - Analysis of paraffin-embedded J5 xenograft, using antibody at 1:500 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778] - Paraffin-embedded mouse colon. CD146 antibody [C1C3] dilution: 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778] - Paraffin-embedded mouse skin. CD146 antibody [C1C3] dilution: 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778] - Mouse placenta. Green: CD146 stained by CD146 antibody [C1C3] diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.