17564-1-AP

antibody from Proteintech Group

Targeting: MCAM CD146, HEMCAM, MelCAM, METCAM, MUC18

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

17564-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#17564-1-AP, RRID:AB_2143373

Product name

CD146/MCAM antibody

Antibody type

Polyclonal

Description

KD/KO validated CD146/MCAM antibody (Cat. #17564-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Establishment of a m(6)A-Related Molecular Pattern in the Prognosis and Immune Infiltration of Osteosarcoma Using Machine Learning and Experiments.

He N, Chen X, Zhang C
International journal of genomics 2026;2026:2000690

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Metabolomics Analysis Reveals Gut Microbiota-Associated Sakuranin Modulates Endometrial Stem Cell Differentiation and Inflammation to Alleviate Pain in Endometriosis.

Shi W, Wang M, Jin Z, Chen X, Li J, Lai H, Li X, Zhong Q, Chen Y, Chen S
Journal of pain research 2026;19:557430

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CD146(+) pericyte-like lung cancer brain metastatic stem cells promote tumor angiogenesis through dual regulatory effects on the VEGF/VEGFR axis.

Liu W, Duan W, Xia S, Liu Y, Chu H, Liang K, Fang S, Lin M, Wei S, You X, Hu Q, Qi J, Wang Q
Theranostics 2026;16(4):1905-1924

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Sal-hDPSCs Transplantation Alleviates Hypoxic-Ischaemic Ferroptosis in Neonatal Rats Via the FSP1-CoQ(10) Axis.

Xu K, Li Y, Gao S, Fang X, Zhang X, Zhang X, Wang F, Wang X
International dental journal 2026 Apr 29;76(4):109573

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Exendin-4 Reduces Senescence of Inflammation-Induced Periodontal Ligament Stem Cells Through SIRT1/Notch1 Signaling.

Xu Y, Zheng J, Liu M, Fu Z, Wang P
Stem cells international 2025;2025:7639451

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Specnuezhenide Alleviates Senile Osteoporosis by Activating TGR5/FXR Signaling in Bone Marrow Mesenchymal Stem Cells and RANKL-Induced Osteoclasts.

Deng X, Lin B, Xiao W, Wang F, Xu P, Wang N
Drug design, development and therapy 2025;19:1595-1608

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Characteristics of lipid metabolism dysfunction in hypertensive disorders of pregnancy.

Wang Z, Zhang L, Zhou X, Zhu S, Li W, Tan W, He Z, Fu Y, Yang Y, Liu Y, Zeng W, Wu J, Wang S, Feng L, Ding W
BMC pregnancy and childbirth 2025 Oct 14;25(1):1080

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Protocatechuic aldehyde promotes diabetic wound healing by enhancing angiogenesis via H3K18 lactylation-mediated Acvr1c expression.

Fan W, You Y, Qu Y, Liu G
Molecular immunology 2025 Nov;187:152-165

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BACH1 deficiency improves placental angiogenesis via SLC25A51-mediated mitochondrial NAD(+) transport in intrahepatic cholestasis of pregnancy.

Li S, Zhu W, Xing Z, Chen D, Zhao H, Zhang Y, Zhang W, Sun J, Wu Y, Ai L, Pang Q
Molecular medicine (Cambridge, Mass.) 2025 May 1;31(1):162

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Senolytic elimination of senescent cells improved periodontal ligament stem cell-based bone regeneration partially through inhibiting YAP.

Jia L, Xiao H, Hao Z, Sun S, Zhao W, Gong Z, Gu W, Wen Y
Biochimica et biophysica acta. Molecular cell research 2025 Mar;1872(3):119921

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Melanoma Cell Adhesion Molecule Plays a Pivotal Role in Proliferation, Migration, Tumor Immune Microenvironment, and Immunotherapy in Colorectal Cancer.

Zhou J, Liu J, Yu Y, Nie H, Hong Y, Ning Y, Yang C, Lai J, Wang H, Tang X, Wang F, Zhao Q
Cancer medicine 2025 Mar;14(5):e70740

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The role of transient receptor potential melastatin channels in compressive force-induced contraction of primary cardiac pericytes.

Methner C, Cilento E, Cao Z, Iliff J, Mishra A, Kaul S
Physiological reports 2025 Jun;13(12):e70396

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Human neural stem cell-derived small extracellular vesicles enhance functional recovery after ischemic stroke through the miR-362-3p/MCAM/Wnt/β-catenin axis.

Zhu Z, Zhang G, Ahmed W, Feng J, Wang C, Zhang Y, Wu G, Hong C, Zhang Q, Feng T, Liu J, Zhao A, Chen L
International immunopharmacology 2025 Jun 17;158:114826

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Spatiotemporally Programming Microenvironment to Recapitulate Endochondral Ossification via Greenhouse-Inspired Bionic Niche.

Liu X, Zhao Y, Wu X, Zhou Y, Liu Y, Wang S, Zhang Y, Yang H, Song F, Huang C
Advanced materials (Deerfield Beach, Fla.) 2025 Jul;37(28):e2504057

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CD142-positive synovial fibroblasts drive meniscus destruction in rheumatoid arthritis.

Sun H, Lin X, Liu F, Zhang Y, Ma J, Huang Z, Huang J, Yao L, Zhang M, Chen M, Xu J, Wei Y, Dai L, Ma R
Nature communications 2025 Jul 28;16(1):6942

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Machine learning developed LKB1-AMPK signaling related signature for prognosis and drug sensitivity in hepatocellular carcinoma.

Li W, Zhu X, Fang J
Scientific reports 2025 Jul 1;15(1):20738

---

Single-cell sequencing reveals MCAM(+) MyCAFs as key pro-angiogenic cells interacting with endothelial cells in solid-type adenoid cystic carcinoma.

Yuan X, Liu Y, Zhang B, Fang Y, Meng L, Chen Z, Zhang H, Mo Y, Jiang W
Cancer cell international 2025 Dec 8;26(1):10

---

CD146(+) CAFs mediate immunosuppression in gastric cancer via COL4A1: potential therapeutic targets.

Chen J, Xu P, Wang Q, Dai J, Liang S, Yang S, Xie G, Li X, Wang Y, Hua H, Ping X, Shen J, Shen L
Journal for immunotherapy of cancer 2025 Dec 21;13(12)

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Cigarette smoke disrupts osteogenic-adipogenic balance via Nrf2/HERC2 axis-driven ferroptosis.

Li W, Song L, Zhang F, Pan Y, Xu W, Huang F, Wang N
Free radical biology & medicine 2025 Dec 1;240:704-716

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The long noncoding RNA lnc-FANCI-2 intrinsically restricts RAS signaling in human papillomavirus type 16-infected cervical cancer cells.

Liu H, Yu L, Majerciak V, Meyer TJ, Yi M, Johnson PF, Cam M, Lowy DR, Zheng ZM
eLife 2025 Aug 29;13

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Rapamycin ameliorates senescence of periodontal ligament stem cells and promotes their osteogenesis via the PI3K/AKT pathway.

Zheng Z, Li Z, Liu X, Liu L, Zhang P, Cui Y, Ding G
International immunopharmacology 2025 Apr 24;153:114517

---

Regeneration process of severed rabbit common calcanean tendons influenced by external compression.

Wu H, Dai X, Huang L, Li X, Wei S, Xu F
Journal of orthopaedic surgery and research 2024 Nov 28;19(1):808

---

Integrin β8 prevents pericyte-myofibroblast transition and renal fibrosis through inhibiting the TGF-β1/TGFBR1/Smad3 pathway in diabetic kidney disease.

Cao Y, Su H, Zeng J, Xie Y, Liu Z, Liu F, Qiu Y, Yi F, Lin J, Hammes HP, Zhang C
Translational research : the journal of laboratory and clinical medicine 2024 Mar;265:36-50

---

Biological characteristics of Muse cells derived from MenSCs and their application in acute liver injury and intracerebral hemorrhage diseases.

Li H, Wei J, Li M, Li Y, Zhang T, Tian J, Liu X, Li K, Lin J
Regenerative therapy 2024 Dec;27:48-62

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Exosomes derived from umbilical cord mesenchymal stem cells promote healing of complex perianal fistulas in rats.

Lu Y, Huangfu S, Ma C, Ding Y, Zhang Y, Zhou C, Liao L, Li M, You J, Chen Y, Wang D, Chen A, Jiang B
Stem cell research & therapy 2024 Dec 29;15(1):414

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The therapeutic value of adipose-derived pericyte transplantation after intracerebral hemorrhage in rats.

Zhang X, Zhou Y, Liu Q, Wang F, Fu L, Wei Y, Ye Y, Guo Y
Journal of molecular histology 2023 Oct;54(5):499-508

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Extrafibrillarly Demineralized Dentin Matrix for Bone Regeneration.

Wu X, Peng W, Liu G, Wang S, Duan B, Yu J, Yang H, Huang C
Advanced healthcare materials 2023 May;12(12):e2202611

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ST6GAL1 inhibits metastasis of hepatocellular carcinoma via modulating sialylation of MCAM on cell surface.

Zou X, Lu J, Deng Y, Liu Q, Yan X, Cui Y, Xiao X, Fang M, Yang F, Sawaki H, Sato T, Tan B, Lu X, Feng B, Kuno A, Narimatsu H, Gao C, Zhang Y
Oncogene 2023 Feb;42(7):516-529

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Rejuvenation of tendon stem/progenitor cells for functional tendon regeneration through platelet-derived exosomes loaded with recombinant Yap1.

Lu J, Yang X, He C, Chen Y, Li C, Li S, Chen Y, Wu Y, Xiang Z, Kang J, Jiang G, Wang C, Diarra MD, He R, Feng G, Yan R
Acta biomaterialia 2023 Apr 15;161:80-99

---

Overexpression of MCAM induced by SMYD2-H3K36me2 in breast cancer stem cell properties.

Li X, Wang Y, Zhang Y, Liu B
Breast cancer (Tokyo, Japan) 2022 Sep;29(5):854-868

---

Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis.

Wu Z, He Y, Chen S, Zhu L, Wang J, Zhang D, Xie J, Zou S, Zhou C
Stem cell research & therapy 2022 Sep 6;13(1):460

---

Pericyte mediates the infiltration, migration, and polarization of macrophages by CD163/MCAM axis in glioblastoma.

Zhang H, Zhang N, Wu W, Wang Z, Dai Z, Liang X, Zhang L, Peng Y, Luo P, Zhang J, Liu Z, Cheng Q, Liu Z
iScience 2022 Sep 16;25(9):104918

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Dental pulp stem cells-based therapy for the oviduct injury via immunomodulation and angiogenesis in vivo.

Luo L, Xing Z, Liao X, Li Y, Luo Y, Ai Y, He Y, Ye Q
Cell proliferation 2022 Oct;55(10):e13293

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Galectin-3 Is a Natural Binding Ligand of MCAM (CD146, MUC18) in Melanoma Cells and Their Interaction Promotes Melanoma Progression.

Pang Y, Maxwell E, Sindrewicz-Goral P, Shapanis A, Li S, Morgan M, Yu LG
Biomolecules 2022 Oct 10;12(10)

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A Hierarchical-Structured Mineralized Nanofiber Scaffold with Osteoimmunomodulatory and Osteoinductive Functions for Enhanced Alveolar Bone Regeneration.

He Y, Tian M, Li X, Hou J, Chen S, Yang G, Liu X, Zhou S
Advanced healthcare materials 2022 Feb;11(3):e2102236

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Mitochondrial calcium uptake regulates tumour progression in embryonal rhabdomyosarcoma.

Chiu HY, Loh AHP, Taneja R
Cell death & disease 2022 Apr 30;13(4):419

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Macropore design of tissue engineering scaffolds regulates mesenchymal stem cell differentiation fate.

Swanson WB, Omi M, Zhang Z, Nam HK, Jung Y, Wang G, Ma PX, Hatch NE, Mishina Y
Biomaterials 2021 May;272:120769

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Co-delivery of fibrin-laminin hydrogel with mesenchymal stem cell spheroids supports skeletal muscle regeneration following trauma.

Genovese P, Patel A, Ziemkiewicz N, Paoli A, Bruns J, Case N, Zustiak SP, Garg K
Journal of tissue engineering and regenerative medicine 2021 Dec;15(12):1131-1143

---

KDM3A/Ets1/MCAM axis promotes growth and metastatic properties in Rhabdomyosarcoma.

Sobral LM, Sechler M, Parrish JK, McCann TS, Jones KL, Black JC, Jedlicka P
Genes & cancer 2020;11(1-2):53-65

---

Salvianolic acid B ameliorates atherosclerosis via inhibiting YAP/TAZ/JNK signaling pathway in endothelial cells and pericytes.

Yang Y, Pei K, Zhang Q, Wang D, Feng H, Du Z, Zhang C, Gao Z, Yang W, Wu J, Li Y
Biochimica et biophysica acta. Molecular and cell biology of lipids 2020 Oct;1865(10):158779

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Immunohistochemical staining with chemokine panel of non-specific colitis predicts future IBD diagnosis.

El-Saka AM, Zamzam YA, Haydara T, Abd-Elsalam S
Cytokine 2020 Mar;127:154935

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Therapeutic Effects of Human Urine-Derived Stem Cells in a Rat Model of Cisplatin-Induced Acute Kidney Injury In Vivo and In Vitro.

Sun B, Luo X, Yang C, Liu P, Yang Y, Dong X, Yang Z, Xu J, Zhang Y, Li L
Stem cells international 2019;2019:8035076

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MicroRNA let-7c-5p promotes osteogenic differentiation of dental pulp stem cells by inhibiting lipopolysaccharide-induced inflammation via HMGA2/PI3K/Akt signal blockade.

Yuan H, Zhao H, Wang J, Zhang H, Hong L, Li H, Che H, Zhang Z
Clinical and experimental pharmacology & physiology 2019 Apr;46(4):389-397

---

The histone demethylase KDM3A, and its downstream target MCAM, promote Ewing Sarcoma cell migration and metastasis.

Sechler M, Parrish JK, Birks DK, Jedlicka P
Oncogene 2017 Jul 20;36(29):4150-4160

---

Role of hypoxia-inducible factor-1α and CD146 in epidermal growth factor receptor-mediated angiogenesis in salivary gland adenoid cystic carcinoma.

Wang WM, Zhao ZL, Zhang WF, Zhao YF, Zhang L, Sun ZJ
Molecular medicine reports 2015 Sep;12(3):3432-3438

---

MCAM is a novel metastasis marker and regulates spreading, apoptosis and invasion of ovarian cancer cells.

Wu Z, Wu Z, Li J, Yang X, Wang Y, Yu Y, Ye J, Xu C, Qin W, Zhang Z
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2012 Oct;33(5):1619-28

---

Comparative proteomic analysis of primary schwann cells and a spontaneously immortalized schwann cell line RSC 96: a comprehensive overview with a focus on cell adhesion and migration related proteins.

Ji Y, Shen M, Wang X, Zhang S, Yu S, Chen G, Gu X, Ding F
Journal of proteome research 2012 Jun 1;11(6):3186-98

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 17564-1-AP( CD146, MCAM Antibody) at dilution of 1:1000

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-CD146, MCAM (IP:17564-1-AP, 3ug; Detection:17564-1-AP 1:500) with HepG2 cells lysate 3600ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human spleen using 17564-1-AP(CD146, MCAM antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The CD146/MCAM antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human CD146/MCAM. This antibody recognizes human,mouse,rat antigen. The CD146/MCAM antibody has been validated for the following applications: ELISA, IHC, IP, WB analysis.

Appendix

17564-1-AP.pdf

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