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Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- MAB7718 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal MCAM/CD146 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse MCAM/CD146 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) MAdCAM-1 is observed, and no cross-reactivity with rmALCAM, rmNCAM, rmL1CAM, rmOCAM, rmTROP-2, recombinant human MCAM, or recombinant rat MCAM is observed.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Precapillary sphincters maintain perfusion in the cerebral cortex.
Grubb S, Cai C, Hald BO, Khennouf L, Murmu RP, Jensen AGK, Fordsmann J, Zambach S, Lauritzen M
Nature communications 2020 Jan 20;11(1):395
Nature communications 2020 Jan 20;11(1):395
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of MCAM/CD146 in NK1.1+ Mouse Splenocytes by Flow Cytometry. Mouse NK1.1+ splenocytes were stained with Rat Anti-Mouse MCAM/CD146 Monoclonal Antibody (Catalog # MAB7718, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
