GTX108777

antibody from GeneTex

Targeting: MCAM CD146, HEMCAM, MelCAM, METCAM, MUC18

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX108777

Provider

GeneTex

Proper citation

GeneTex Cat#GTX108777, RRID:AB_10729041

Product name

CD146 antibody [C1C3]

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: HCT116 5% SDS PAGE GTX108777 diluted at 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

CD146 antibody [C1C3] detects CD146 protein by western blot analysis.A. 50 £gg rat liver extract7.5 % SDS-PAGECD146 antibody [C1C3] (GTX108777) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

CD146 antibody [C1C3] detects CD146 protein by Western blot analysis.A. 30 £gg HUVEC whole cell lysate/extract7.5 % SDS-PAGECD146 antibody [C1C3] (GTX108777) dilution: 1:10000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

CD146 antibody [C1C3] detects CD146 protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: HUVEC cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD146 protein stained by CD146 antibody [C1C3] (GTX108777) diluted at 1:500.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD146(GTX108777) antibody at 1:500 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

CD146 antibody [C1C3] detects CD146 protein at membrane on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. CD146 antibody [C1C3] (GTX108777) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

CD146 antibody [C1C3] detects CD146 protein at membrane on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. CD146 antibody [C1C3] (GTX108777) dilution: 1:500.