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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- 35-7800 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD146 Monoclonal Antibody (OJ79)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OJ79
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references Immunocytochemistry of CD146 is useful to discriminate between malignant pleural mesothelioma and reactive mesothelium.
Sato A, Torii I, Okamura Y, Yamamoto T, Nishigami T, Kataoka TR, Song M, Hasegawa S, Nakano T, Kamei T, Tsujimura T
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2010 Nov;23(11):1458-66
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2010 Nov;23(11):1458-66
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CD146 Monoclonal Antibody (OJ79) (Product # 35-7800) and a 110 kDa band corresponding to cell surface glycoprotein MUC18 was observed across cell lines and tissues. Whole cell extracts (30 µg lysate) of SK-MEL-28 (Lane 1), SK-BR-3 (Lane 2), HUVEC (Lane 3), T-47D (Lane 4), MCF7 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MUC18 was performed by loading 20 µg of HeLa (lane1) and HUVEC (lane2) cell lysate using Novex®NuPAGE® 12% Bis-Tris gel (Product # NP0342BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and Proteins were transferred to a PVDF membrane and blocked with 5% skim milk for 1 hour at room temperature. MUC18 was detected at ~110 kDa using MUC 18 Mouse Monoclonal Antibody (Product # 35-7800) at 0.5-2 µg/mL in 2.5% skim milk at 4°C overnight on a rocking platform. Goat Anti-Mouse IgG - HRP Secondary Antibody (Product # 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
