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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [7]
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- Product number
- TA303592 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA303592, RRID:AB_2143390
- Product name
- Rabbit monoclonal antibody against CD146 (EPR3208 )
- Antibody type
- Monoclonal
- Description
- Rabbit monoclonal antibody against CD146 (EPR3208 )
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- MCAM
- Isotype
- IgG
- Antibody clone number
- EPR3208
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry/ Immunofluorescence - CD146 antibody [EPR3208]; TA303592 staining CD146 in murine bone marrow cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in methanol and then blocked using 5% serum for 2 hours at 25°C. Samples were then incubated with the primary antibody at 1/250 for 12 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor 488 (green) used at a 1/500 dilution. Counterstained with DAPI.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD146 antibody [EPR3208]; TA303592 at 1/250 dilution staining CD146 in melanoma tissue, using a HRP/AP polymerized secondary antibody.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen sections) - CD146 antibody [EPR3208]; TA303592 staining CD146 in mouse spleen tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with acetone and blocked with 5% serum for 2 hours at 25°C. The sample was incubated with primary antibody (1/250 in PBS-Tween) at 4°C for 12 hours. An Alexa Fluor488-conjugated Goat polyclonal to rabbit IgG (1/250) was used as secondary antibody. Nuclear staining was with DAPI.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD146 antibody [EPR3208](TA303592); TA303592 showing positive staining in Breast carcinoma vessels tissue.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD146 antibody [EPR3208](TA303592); TA303592 showing positive staining in Urinary bladder transitional carcinoma vessels tissue.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD146 antibody [EPR3208](TA303592); TA303592 showing positive staining in Glioma vessels tissue.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD146 antibody [EPR3208](TA303592); TA303592 showing positive staining in Normal tonsil tissue.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD146 antibody [EPR3208](TA303592); TA303592 showing positive staining in Normal spleen tissue.
- Validation comment
- IHC
