TA322494

antibody from OriGene

Targeting: MCAM CD146, HEMCAM, MelCAM, METCAM, MUC18

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA322494

Provider

OriGene

Product name

Anti-MCAM Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide peptide corresponding to a region derived from 345-358 amino acids of human melanoma cell adhesion molecule

Description

Anti-MCAM Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µl

Concentration

3.4mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 72 kDa . Positive control: Human fetal intestines tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/1700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm, cell membrane. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using MCAM antibody at dilution 1/100, on the right is treated with the synthetic peptide. (Original magnification:x200)

Validation comment

IHC