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- Antibody Data
- Antigen structure
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- Western blot [2]
- Immunohistochemistry [14]
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- Product number
- UM800052 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM800052, RRID:AB_2629163
- Product name
- CD146 (MCAM) mouse monoclonal antibody,clone UMAB155
- Antibody type
- Monoclonal
- Description
- CD146 (MCAM) mouse monoclonal antibody,clone UMAB155
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- MCAM
- Isotype
- IgG
- Antibody clone number
- UMAB155
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of MALME3M cell lysate (35ug) by using anti-MCAM monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil using anti-MCAMmouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human bladder tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human melanoma using anti- CD146 (MCAM) clone UMAB155 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800052 requires HIER with with Accel 3in1 EDTA solution ph8.7 at 110C for 3 min using pressure chamber/cooker. The tumor cells shows strong membraneous and cytoplasmic staining.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue using anti-CD146 clone UMAB155 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, UM800052)(1:300).
- Validation comment
- IHC
