MAB932-100

antibody from R&D Systems

Targeting: MCAM CD146, HEMCAM, MelCAM, METCAM, MUC18

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

MAB932-100

Provider

R&D Systems

Product name

Human MCAM/CD146 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human MCAM in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEpCAM, rhNCAM-L1, recombinant mouse (rm) OCAM, or rmMAdCAM-1 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

AAA20824


Isotype

IgG

Antibody clone number

128018

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Fluoxetine induces direct inhibitory effects on mesenchymal stem cell‑derived osteoprogenitor cells independent of serotonin concentration.

Koura SM, Salama M, El-Hussiny M, Khalil MEA, Lotfy A, Hassan SA, Gad Elhak SA, Sobh MA
Molecular medicine reports 2019 Apr;19(4):2611-2619

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Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles.

Zaborowski MP, Lee K, Na YJ, Sammarco A, Zhang X, Iwanicki M, Cheah PS, Lin HY, Zinter M, Chou CY, Fulci G, Tannous BA, Lai CP, Birrer MJ, Weissleder R, Lee H, Breakefield XO
Cell reports 2019 Apr 2;27(1):255-268.e6

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Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke.

Tan C, Shichinohe H, Wang Z, Hamauchi S, Abumiya T, Nakayama N, Kazumata K, Ito T, Kudo K, Takamoto S, Houkin K
Stem cells international 2016;2016:6104780

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Choice of xenogenic-free expansion media significantly influences the myogenic differentiation potential of human bone marrow-derived mesenchymal stromal cells.

Brun J, Abruzzese T, Rolauffs B, Aicher WK, Hart ML
Cytotherapy 2016 Mar;18(3):344-59

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Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor.

Im H, Shao H, Park YI, Peterson VM, Castro CM, Weissleder R, Lee H
Nature biotechnology 2014 May;32(5):490-5

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human MCAM/CD146 by Western Blot. Western blot shows lysate of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MCAM/CD146 Monoclonal Antibody (Catalog # MAB932) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MCAM/CD146 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

MCAM/CD146 in BG01V Human Embryonic Stem Cells. MCAM/CD146 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human MCAM/CD146 Monoclonal Antibody (Catalog # MAB932) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.