Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- FAB932F-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human MCAM/CD146 Fluorescein-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human MCAM/CD146 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) ALCAM, rhBCAM, rhEpCAM, rhNCAM-L1, recombinant mouse (rm)OCAM, or rmMAdCAM-1.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
AAA20824
- Isotype
- IgG
- Antibody clone number
- 128018
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R.
Late outgrowth endothelial cells resemble mature endothelial cells and are not derived from bone marrow.
Oxygen tension modifies the 'stemness' of human cord blood-derived stem cells.
High levels of β-catenin signaling reduce osteogenic differentiation of stem cells in inflammatory microenvironments through inhibition of the noncanonical Wnt pathway.
Hyperoxia disrupts vascular endothelial growth factor-nitric oxide signaling and decreases growth of endothelial colony-forming cells from preterm infants.
Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Höller C, Wacheck V, Pehamberger H, Valent P
PloS one 2014;9(1):e84417
PloS one 2014;9(1):e84417
Late outgrowth endothelial cells resemble mature endothelial cells and are not derived from bone marrow.
Tura O, Skinner EM, Barclay GR, Samuel K, Gallagher RC, Brittan M, Hadoke PW, Newby DE, Turner ML, Mills NL
Stem cells (Dayton, Ohio) 2013 Feb;31(2):338-48
Stem cells (Dayton, Ohio) 2013 Feb;31(2):338-48
Oxygen tension modifies the 'stemness' of human cord blood-derived stem cells.
Buchheiser A, Houben AP, Bosch J, Marbach J, Liedtke S, Kögler G
Cytotherapy 2012 Sep;14(8):967-82
Cytotherapy 2012 Sep;14(8):967-82
High levels of β-catenin signaling reduce osteogenic differentiation of stem cells in inflammatory microenvironments through inhibition of the noncanonical Wnt pathway.
Liu N, Shi S, Deng M, Tang L, Zhang G, Liu N, Ding B, Liu W, Liu Y, Shi H, Liu L, Jin Y
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2011 Sep;26(9):2082-95
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2011 Sep;26(9):2082-95
Hyperoxia disrupts vascular endothelial growth factor-nitric oxide signaling and decreases growth of endothelial colony-forming cells from preterm infants.
Fujinaga H, Baker CD, Ryan SL, Markham NE, Seedorf GJ, Balasubramaniam V, Abman SH
American journal of physiology. Lung cellular and molecular physiology 2009 Dec;297(6):L1160-9
American journal of physiology. Lung cellular and molecular physiology 2009 Dec;297(6):L1160-9
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of MCAM/CD146 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human MCAM/CD146 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB932F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.