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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- AF3250 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat MCAM/CD146 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects rat MCAM/CD146 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human MCAM is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9EPF2- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of MCAM/CD146 in PC-12 Rat Cell Line by Flow Cytometry. PC-12 rat adrenal pheochromocytoma cell line was stained with Goat Anti-Rat MCAM/CD146 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3250, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
