BAF3250

antibody from R&D Systems

Targeting: MCAM CD146, HEMCAM, MelCAM, METCAM, MUC18

Western blot (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

BAF3250

Provider

R&D Systems

Product name

Rat MCAM/CD146 Biotinylated Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects rat MCAM/CD146 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human MCAM is observed.

Reactivity

Rat

Host

Goat

Conjugate

Biotin

Antigen sequence

Q9EPF2


Isotype

IgG

Vial size

50 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of MCAM/CD146 in PC-12 Rat Cell Line by Flow Cytometry. PC-12 rat adrenal pheo-chromocytoma cell line was stained with Rat MCAM/CD146 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3250, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).