Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 48-1469-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD146 Monoclonal Antibody (P1H12), eFluor 450, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody P1H12 recognizes CD146 also known as MUC18, s-endo, Endo-CAM and Mel-CAM, which is a member of the Ig superfamily of proteins. The expression of CD146 is found on endothelial cells, bone marrow fibroblasts and some tumors (especially melanoma). Recently mesenchymal stromal cells and endometrial stromal cells have also been shown to express CD146. The presence of CD146 on circulating blood cells have been confined to a subset of T cells rather than circulating endothelial cells, as expression of other endothelial markers (CD31 and CD51/61) is negative. Expression can be found on activated lymphocytes. The protein is heavily glycosylated with more than 50% of the mass from carbohydrates. The antibody P1H12 has been reported to crossreact to mouse, rabbit, canine, but not rat. Applications Reported: This P1H12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This P1H12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse, Canine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- P1H12
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Molecular Programming of Perivascular Stem Cell Precursors.
Tumor-associated B-cells induce tumor heterogeneity and therapy resistance.
Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification.
Application of anodized titanium for enhanced recruitment of endothelial progenitor cells.
Canine hemangiosarcoma originates from hematopoietic precursors with potential for endothelial differentiation.
CD146 (Mel-CAM), an adhesion marker of endothelial cells, is a novel marker of lymphocyte subset activation in normal peripheral blood.
Identification and functional assessment of endothelial P1H12.
Circulating activated endothelial cells in sickle cell anemia.
Yianni V, Sharpe PT
Stem cells (Dayton, Ohio) 2018 Dec;36(12):1890-1904
Stem cells (Dayton, Ohio) 2018 Dec;36(12):1890-1904
Tumor-associated B-cells induce tumor heterogeneity and therapy resistance.
Somasundaram R, Zhang G, Fukunaga-Kalabis M, Perego M, Krepler C, Xu X, Wagner C, Hristova D, Zhang J, Tian T, Wei Z, Liu Q, Garg K, Griss J, Hards R, Maurer M, Hafner C, Mayerhöfer M, Karanikas G, Jalili A, Bauer-Pohl V, Weihsengruber F, Rappersberger K, Koller J, Lang R, Hudgens C, Chen G, Tetzlaff M, Wu L, Frederick DT, Scolyer RA, Long GV, Damle M, Ellingsworth C, Grinman L, Choi H, Gavin BJ, Dunagin M, Raj A, Scholler N, Gross L, Beqiri M, Bennett K, Watson I, Schaider H, Davies MA, Wargo J, Czerniecki BJ, Schuchter L, Herlyn D, Flaherty K, Herlyn M, Wagner SN
Nature communications 2017 Sep 19;8(1):607
Nature communications 2017 Sep 19;8(1):607
Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification.
Agarwal S, Loder SJ, Sorkin M, Li S, Shrestha S, Zhao B, Mishina Y, James AW, Levi B
Stem cells (Dayton, Ohio) 2016 Jun;34(6):1692-701
Stem cells (Dayton, Ohio) 2016 Jun;34(6):1692-701
Application of anodized titanium for enhanced recruitment of endothelial progenitor cells.
Moradi I, Behjati M, Kazemi M
Nanoscale research letters 2012 Jun 7;7(1):298
Nanoscale research letters 2012 Jun 7;7(1):298
Canine hemangiosarcoma originates from hematopoietic precursors with potential for endothelial differentiation.
Lamerato-Kozicki AR, Helm KM, Jubala CM, Cutter GC, Modiano JF
Experimental hematology 2006 Jul;34(7):870-8
Experimental hematology 2006 Jul;34(7):870-8
CD146 (Mel-CAM), an adhesion marker of endothelial cells, is a novel marker of lymphocyte subset activation in normal peripheral blood.
Elshal MF, Khan SS, Takahashi Y, Solomon MA, McCoy JP Jr
Blood 2005 Oct 15;106(8):2923-4
Blood 2005 Oct 15;106(8):2923-4
Identification and functional assessment of endothelial P1H12.
Solovey AN, Gui L, Chang L, Enenstein J, Browne PV, Hebbel RP
The Journal of laboratory and clinical medicine 2001 Nov;138(5):322-31
The Journal of laboratory and clinical medicine 2001 Nov;138(5):322-31
Circulating activated endothelial cells in sickle cell anemia.
Solovey A, Lin Y, Browne P, Choong S, Wayner E, Hebbel RP
The New England journal of medicine 1997 Nov 27;337(22):1584-90
The New England journal of medicine 1997 Nov 27;337(22):1584-90
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0038-42) and Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-CD146 eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.