50-1469-41

antibody from Invitrogen Antibodies
Targeting: MCAM CD146, HEMCAM, MelCAM, METCAM, MUC18
Provider product page for 50-1469-41

Antibody data

Product number
50-1469-41 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-CD146 Monoclonal Antibody (P1H12), eFluor 660, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The monoclonal antibody P1H12 recognizes CD146 also known as MUC18, s-endo, Endo-CAM and Mel-CAM, which is a member of the Ig superfamily of proteins. The expression of CD146 is found on endothelial cells, bone marrow fibroblasts and some tumors (especially melanoma). Recently mesenchymal stromal cells and endometrial stromal cells have also been shown to express CD146. The presence of CD146 on circulating blood cells have been confined to a subset of T cells rather than circulating endothelial cells, as expression of other endothelial markers (CD31 and CD51/61) is negative. Expression can be found on activated lymphocytes. The protein is heavily glycosylated with more than 50% of the mass from carbohydrates. The antibody P1H12 has been reported to crossreact to mouse, rabbit, canine, but not rat. Applications Reported: This P1H12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This P1H12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human, Mouse, Canine, Rabbit
Host
Mouse
Isotype
IgG
Antibody clone number
P1H12
Vial size
25 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
MCAM protein structure - 50-1469-41 shown in red.