17564-1-AP
antibody from Invitrogen Antibodies
Targeting: MCAM
CD146, HEMCAM, MelCAM, METCAM, MUC18
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [18]
- Flow cytometry [1]
- Other assay [2]
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Validation data
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- Product number
- 17564-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD146/MCAM Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: GEAEQPAPE LVEVEVGSTA LLKCGLSQSQ GNLSHVDWFS VHKEKRTLIF RVRQGQGQSE PGEYEQRLSL QDRGATLALT QVTPQDERIF LCQGKRPRSQ EYRIQLRVYK APEEPNIQVN PLGIPVNSKE PEEVATCVGR NGYPIPQVIW YKNGRPLKEE KNRVHIQSSQ TVESSGLYTL QSILKAQLVK EDKDAQFYCE LNYRLPSGNH MKESREVTVP VFYPTEKVWL EVEPVGMLKE GDRVEIRCLA DGNPPPHFSI SKQNPSTREA EEETTNDNGV LVLEPARKEH SGRYECQGLD LDTMISLLSE PQELLVNYVS DVRVSP (26-350 aa encoded by BC056418)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A375 cells were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 17564-1-AP ( CD146/MCAM antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 17564-1-AP ( CD146/MCAM antibody at dilution of 1:600 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 17564-1-AP ( CD146/MCAM antibody at dilution of 1:600 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 17564-1-AP (CD146/MCAM antibody at dilution of 1:600 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 17564-1-AP (CD146/MCAM antibody at dilution of 1:600 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:100 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HUVEC cells were stained with 0.2ug CD146/MCAM antibody (17564-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CD146/MCAM (IP:17564-1-AP, 4ug; Detection:17564-1-AP 1:1500) with HeLa cells lysate 4000ug.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CD146/MCAM (IP:17564-1-AP, 3 ug; Detection:17564-1-AP 1:500) with HepG2 cells lysate 3600 ug.