Explore
Validate
LearnNBP1-43346-0.1mg
antibody from Novus Biologicals
Targeting: MCAM
CD146, HEMCAM, MelCAM, METCAM, MUC18
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-43346-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-43346-0.1mg, RRID:AB_10006724
- Product name
- Mouse Monoclonal MCAM/CD146 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human, Mouse, Rat, Canine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C. Do not freeze.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: MCAM/CD146 Antibody (P1H12) [NBP1-43346] - Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with 0.125 ug of Mouse IgG1 K Isotype Control Purified (blue histogram) or 0.125 ug of Anti-CD146 Purified (purple histogram) followed by F(ab')2 Anti-Mouse IgG PE.Total viable cells were used for analysis.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: MCAM/CD146 Antibody (P1H12) [NBP1-43346] - Analysis using the FITC conjugate of NBP1-43346. Left: Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with Mouse IgG1 K Isotype Control FITC (blue histogram) or Anti-CD146 FITC (purple histogram). Right: Staining of normal human peripheral blood cells with Anti-Human CD3 APC and Anti-CD146 FITC.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: MCAM/CD146 Antibody (P1H12) [NBP1-43346] - A surface stain was performed on SK-MEL-28 cells with MCAM/CD146 Antibody (P1H12) NBP1-43346PCP (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to PerCP.
