Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [7]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-88132 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88132, RRID:AB_11008437
- Product name
- Rabbit Polyclonal MCAM/CD146 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human MCAM/CD146 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCAM/CD146 Antibody [NBP1-88132] - Analysis in human cell line WM-115.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCAM/CD146 Antibody [NBP1-88132] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-MCAM antibody. Corresponding MCAM RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP1-88132] - Staining of human colon shows strong positivity in endothelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP1-88132] - Staining of human colon shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP1-88132] - Staining of human liver shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP1-88132] - Staining of human pancreas shows weak cytoplasmic positivity in endothelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP1-88132] - Staining in human colon and liver tissues using anti-MCAM antibody. Corresponding MCAM RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP1-88132] - Staining of human placenta shows moderate cytoplasmic positivity in endothelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP1-88132] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.