M03598
antibody from Boster Biological Technology
Targeting: ATP5F1A
ATP5A, ATP5A1, ATP5AL2, ATPM, hATP1, OMR, ORM
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- M03598 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for ATP5A/ATP5A1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat ATP5A/ATP5A1 information: Molecular Weight: 59751 MW; Subcellular Localization: Mitochondrion inner membrane . Cell membrane; Peripheral membrane protein; Extracellular side . Colocalizes with HRG on the cell surface of T-cells (PubMed:19285951); Tissue Specificity: Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- AAFC-1
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of ATP5A1 expression in HepG2 cell lysate; (M03598). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5A1 monoclonal antibody (Catalog # M03598) overnight at 4
- Additional image