M03598

antibody from Boster Biological Technology

Targeting: ATP5F1A ATP5A, ATP5A1, ATP5AL2, ATPM, hATP1, OMR, ORM

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

M03598

Provider

Boster Biological Technology

Product name

Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for ATP5A/ATP5A1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat ATP5A/ATP5A1 information: Molecular Weight: 59751 MW; Subcellular Localization: Mitochondrion inner membrane . Cell membrane; Peripheral membrane protein; Extracellular side . Colocalizes with HRG on the cell surface of T-cells (PubMed:19285951); Tissue Specificity: Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

AAFC-1

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of ATP5A1 expression in HepG2 cell lysate; (M03598). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5A1 monoclonal antibody (Catalog # M03598) overnight at 4

Additional image