14676-1-AP

antibody from Proteintech Group

Targeting: ATP5F1A ATP5A, ATP5A1, ATP5AL2, ATPM, hATP1, OMR, ORM

Provider product page for 14676-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [57]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 14676-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#14676-1-AP, RRID:AB_2061761
Product name: ATP5A1 antibody
Antibody type: Polyclonal
Description: ATP5A1 antibody (Cat. #14676-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

ATP5F1A protein structure - 14676-1-AP shown in red.

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Mitochondrial ROS promote mitochondrial dysfunction and inflammation in ischemic acute kidney injury by disrupting TFAM-mediated mtDNA maintenance.

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Biochanin A Inhibits Glioblastoma Growth via Restricting Glycolysis and Mitochondrial Oxidative Phosphorylation.

Dong Q, Li Q, Duan L, Yin H, Wang X, Liu Y, Wang B, Li K, Yao X, Yuan G, Pan Y
Frontiers in oncology 2021;11:652008

Punicalagin Protects Against Diabetic Cardiomyopathy by Promoting Opa1-Mediated Mitochondrial Fusion via Regulating PTP1B-Stat3 Pathway.

Fu F, Liu C, Shi R, Li M, Zhang M, Du Y, Wang Q, Li J, Wang G, Pei J, Ding M
Antioxidants & redox signaling 2021 Sep;35(8):618-641

Celecoxib reverses the glioblastoma chemo-resistance to temozolomide through mitochondrial metabolism.

Yin D, Jin G, He H, Zhou W, Fan Z, Gong C, Zhao J, Xiong H
Aging 2021 Sep 8;13(17):21268-21282

Metabolic targeting of cancer by a ubiquinone uncompetitive inhibitor of mitochondrial complex I.

Jain S, Hu C, Kluza J, Ke W, Tian G, Giurgiu M, Bleilevens A, Campos AR, Charbono A, Stickeler E, Maurer J, Holinski-Feder E, Vaisburg A, Bureik M, Luo G, Marchetti P, Cheng Y, Wolf DA
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Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery.

Schäfer JA, Bozkurt S, Michaelis JB, Klann K, Münch C
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Effects of photoperiod on morphology and function in testis and epididymis of Cricetulus barabensis.

Mou J, Xu J, Wang Z, Wang C, Yang X, Wang X, Xue H, Wu M, Xu L
Journal of cellular physiology 2021 Mar;236(3):2109-2125

AMPK-dependent and -independent coordination of mitochondrial function and muscle fiber type by FNIP1.

Xiao L, Liu J, Sun Z, Yin Y, Mao Y, Xu D, Liu L, Xu Z, Guo Q, Ding C, Sun W, Yang L, Zhou Z, Zhou D, Fu T, Zhou W, Zhu Y, Chen XW, Li JZ, Chen S, Xie X, Gan Z
PLoS genetics 2021 Mar;17(3):e1009488

Parkin-independent mitophagy via Drp1-mediated outer membrane severing and inner membrane ubiquitination.

Oshima Y, Cartier E, Boyman L, Verhoeven N, Polster BM, Huang W, Kane M, Lederer WJ, Karbowski M
The Journal of cell biology 2021 Jun 7;220(6)

OMA1 reprograms metabolism under hypoxia to promote colorectal cancer development.

Wu Z, Zuo M, Zeng L, Cui K, Liu B, Yan C, Chen L, Dong J, Shangguan F, Hu W, He H, Lu B, Song Z
EMBO reports 2021 Jan 7;22(1):e50827

P2X7 receptor signaling promotes inflammation in renal parenchymal cells suffering from ischemia-reperfusion injury.

Qian Y, Qian C, Xie K, Fan Q, Yan Y, Lu R, Wang L, Zhang M, Wang Q, Mou S, Dai H, Ni Z, Pang H, Gu L
Cell death & disease 2021 Jan 27;12(1):132

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Kong XT, Wang Z, Mou JJ, Li CS, Xue HL, Wu M, Chen L, Xu JH, Xu LX
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 2021 Apr-May;253:110547

RGS12 is required for the maintenance of mitochondrial function during skeletal development.

Yuan G, Yang S, Liu M, Yang S
Cell discovery 2020;6:59

Mitochondrial Transcription Factor A added to Osteocytes in a Stressed Environment has a Cytoprotective Effect.

Ueda S, Shimasaki M, Ichiseki T, Hirata H, Kawahara N, Ueda Y
International journal of medical sciences 2020;17(9):1293-1299

Drp1-mediated mitochondrial fission contributes to baicalein-induced apoptosis and autophagy in lung cancer via activation of AMPK signaling pathway.

Deng X, Liu J, Liu L, Sun X, Huang J, Dong J
International journal of biological sciences 2020;16(8):1403-1416

The effect of autophagy and mitochondrial fission on Harderian gland is greater than apoptosis in male hamsters during different photoperiods.

Xu JH, Wang Z, Mou JJ, Zhao XY, Geng XC, Wu M, Xue HL, Chen L, Xu LX
PloS one 2020;15(11):e0241561

Photoperiod Affects Harderian Gland Morphology and Secretion in Female Cricetulus barabensis: Autophagy, Apoptosis, and Mitochondria.

Wang Z, Xu JH, Mou JJ, Kong XT, Wu M, Xue HL, Xu LX
Frontiers in physiology 2020;11:408

Taurine Inhibits Glucocorticoid-Induced Bone Mitochondrial Injury, Preventing Osteonecrosis in Rabbits and Cultured Osteocytes.

Hirata H, Ueda S, Ichiseki T, Shimasaki M, Ueda Y, Kaneuji A, Kawahara N
International journal of molecular sciences 2020 Sep 20;21(18)

JNK signalling regulates antioxidant responses in neurons.

Ugbode C, Garnham N, Fort-Aznar L, Evans GJO, Chawla S, Sweeney ST
Redox biology 2020 Oct;37:101712

A Regulation Loop between YAP and NR4A1 Balances Cell Proliferation and Apoptosis.

He L, Yuan L, Yu W, Sun Y, Jiang D, Wang X, Feng X, Wang Z, Xu J, Yang R, Zhang W, Feng H, Chen HZ, Zeng YA, Hui L, Wu Q, Zhang Y, Zhang L
Cell reports 2020 Oct 20;33(3):108284

Novel ultrastructural findings on cardiac mitochondria of huddling Brandt's voles in mild cold environment.

Wang Z, Xu JH, Mou JJ, Kong XT, Zou JW, Xue HL, Wu M, Xu LX
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 2020 Nov;249:110766

PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis.

Yan C, Gong L, Chen L, Xu M, Abou-Hamdan H, Tang M, Désaubry L, Song Z
Autophagy 2020 Mar;16(3):419-434

Romo1 is involved in the immune response of glioblastoma by regulating the function of macrophages.

Sun G, Cao Y, Qian C, Wan Z, Zhu J, Guo J, Shi L
Aging 2020 Jan 16;12(2):1114-1127

BRD4 inhibition by JQ1 prevents high-fat diet-induced diabetic cardiomyopathy by activating PINK1/Parkin-mediated mitophagy in vivo.

Mu J, Zhang D, Tian Y, Xie Z, Zou MH
Journal of molecular and cellular cardiology 2020 Dec;149:1-14

A cross-talk between epithelium and endothelium mediates human alveolar-capillary injury during SARS-CoV-2 infection.

Wang P, Luo R, Zhang M, Wang Y, Song T, Tao T, Li Z, Jin L, Zheng H, Chen W, Zhao M, Zheng Y, Qin J
Cell death & disease 2020 Dec 8;11(12):1042

The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.

Zheng L, Shu WJ, Li YM, Mari M, Yan C, Wang D, Yin ZH, Jiang W, Zhou Y, Okamoto K, Reggiori F, Klionsky DJ, Song Z, Du HN
Autophagy 2020 Aug;16(8):1366-1379

Artemisinin suppresses hepatocellular carcinoma cell growth, migration and invasion by targeting cellular bioenergetics and Hippo-YAP signaling.

Li Y, Lu J, Chen Q, Han S, Shao H, Chen P, Jin Q, Yang M, Shangguan F, Fei M, Wang L, Liu Y, Liu N, Lu B
Archives of toxicology 2019 Nov;93(11):3367-3383

Insufficient fumarase contributes to hypertension by an imbalance of redox metabolism in Dahl salt-sensitive rats.

Zheng X, Chen M, Li X, Yang P, Zhao X, Ouyang Y, Yang Z, Liang M, Hou E, Tian Z
Hypertension research : official journal of the Japanese Society of Hypertension 2019 Nov;42(11):1672-1682

Benzoylaconine induces mitochondrial biogenesis in mice via activating AMPK signaling cascade.

Deng XH, Liu JJ, Sun XJ, Dong JC, Huang JH
Acta pharmacologica Sinica 2019 May;40(5):658-665

TDP-43 induces mitochondrial damage and activates the mitochondrial unfolded protein response.

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PLoS genetics 2019 May;15(5):e1007947

The 3'UTR of human MAVS mRNA contains multiple regulatory elements for the control of protein expression and subcellular localization.

Xu L, Peng L, Gu T, Yu D, Yao YG
Biochimica et biophysica acta. Gene regulatory mechanisms 2019 Jan;1862(1):47-57

Promotion of Mitochondrial Biogenesis via Activation of AMPK-PGC1ɑ Signaling Pathway by Ginger (Zingiber officinale Roscoe) Extract, and Its Major Active Component 6-Gingerol.

Deng X, Zhang S, Wu J, Sun X, Shen Z, Dong J, Huang J
Journal of food science 2019 Aug;84(8):2101-2111

Avenanthramide A triggers potent ROS-mediated anti-tumor effects in colorectal cancer by directly targeting DDX3.

Fu R, Yang P, Li Z, Liu W, Amin S, Li Z
Cell death & disease 2019 Aug 7;10(8):593

Energy Metabolism in the Bone is Associated with Histomorphometric Changes in Rats with Hyperthyroidism.

Hu Z, Du M, Lai W, Liang Y, Liu Q, Mo Y, Bei J, Li S, Yang Y, Xu J, Cui L
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;46(4):1471-1482

AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.

Ni Z, He J, Wu Y, Hu C, Dai X, Yan X, Li B, Li X, Xiong H, Li Y, Li S, Xu L, Li Y, Lian J, He F
Autophagy 2018;14(4):685-701

Mitophagy Directs Muscle-Adipose Crosstalk to Alleviate Dietary Obesity.

Fu T, Xu Z, Liu L, Guo Q, Wu H, Liang X, Zhou D, Xiao L, Liu L, Liu Y, Zhu MS, Chen Q, Gan Z
Cell reports 2018 May 1;23(5):1357-1372

Leber's hereditary optic neuropathy (LHON)-associated ND5 12338T > C mutation altered the assembly and function of complex I, apoptosis and mitophagy.

Zhang J, Ji Y, Lu Y, Fu R, Xu M, Liu X, Guan MX
Human molecular genetics 2018 Jun 1;27(11):1999-2011

Serine protease PRSS55 is crucial for male mouse fertility via affecting sperm migration and sperm-egg binding.

Shang X, Shen C, Liu J, Tang L, Zhang H, Wang Y, Wu W, Chi J, Zhuang H, Fei J, Wang Z
Cellular and molecular life sciences : CMLS 2018 Dec;75(23):4371-4384

YiQiFuMai Powder Injection Protects against Ischemic Stroke via Inhibiting Neuronal Apoptosis and PKCδ/Drp1-Mediated Excessive Mitochondrial Fission.

Xu Y, Wang Y, Wang G, Ye X, Zhang J, Cao G, Zhao Y, Gao Z, Zhang Y, Yu B, Kou J
Oxidative medicine and cellular longevity 2017;2017:1832093

Protein profiling and functional analysis of liver mitochondria from rats with nonalcoholic steatohepatitis.

You Y, Zhang Y, Lu Y, Hu K, Qu X, Liu Y, Lu B, Jin L
Molecular medicine reports 2017 Sep;16(3):2379-2388

Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.

Wu L, Guo X, Lyu Y, Clarke SL, Lucas EA, Smith BJ, Hildebrand D, Wang W, Medeiros DM, Shen X, Lin D
Scientific reports 2017 Nov 7;7(1):14624

Lack of β, β-carotene-9', 10'-oxygenase 2 leads to hepatic mitochondrial dysfunction and cellular oxidative stress in mice.

Wu L, Guo X, Hartson SD, Davis MA, He H, Medeiros DM, Wang W, Clarke SL, Lucas EA, Smith BJ, von Lintig J, Lin D
Molecular nutrition & food research 2017 May;61(5)

DJ-1 modulates mitochondrial response to oxidative stress: clues from a novel diagnosis of PARK7.

Di Nottia M, Masciullo M, Verrigni D, Petrillo S, Modoni A, Rizzo V, Di Giuda D, Rizza T, Niceta M, Torraco A, Bianchi M, Santoro M, Bentivoglio AR, Bertini E, Piemonte F, Carrozzo R, Silvestri G
Clinical genetics 2017 Jul;92(1):18-25

Exercise Inducible Lactate Dehydrogenase B Regulates Mitochondrial Function in Skeletal Muscle.

Liang X, Liu L, Fu T, Zhou Q, Zhou D, Xiao L, Liu J, Kong Y, Xie H, Yi F, Lai L, Vega RB, Kelly DP, Smith SR, Gan Z
The Journal of biological chemistry 2016 Dec 2;291(49):25306-25318

Inhibition of Neuroblastoma Tumor Growth by Ketogenic Diet and/or Calorie Restriction in a CD1-Nu Mouse Model.

Morscher RJ, Aminzadeh-Gohari S, Feichtinger RG, Mayr JA, Lang R, Neureiter D, Sperl W, Kofler B
PloS one 2015;10(6):e0129802

Interaction of cellular proteins with BCL-xL targeted to cytoplasmic inclusion bodies in adenovirus infected cells.

Subramanian T, Vijayalingam S, Kuppuswamy M, Chinnadurai G
Virology 2015 Sep;483:21-31

Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure.

Ding C, Wu Z, Huang L, Wang Y, Xue J, Chen S, Deng Z, Wang L, Song Z, Chen S
Scientific reports 2015 Nov 4;5:16064

Lipolytic inhibitor G0/G1 switch gene 2 inhibits reactive oxygen species production and apoptosis in endothelial cells.

Wang Y, Zhang Y, Zhu Y, Zhang P
American journal of physiology. Cell physiology 2015 Mar 15;308(6):C496-504

Evaluation of intramitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation.

Kioka H, Kato H, Fujikawa M, Tsukamoto O, Suzuki T, Imamura H, Nakano A, Higo S, Yamazaki S, Matsuzaki T, Takafuji K, Asanuma H, Asakura M, Minamino T, Shintani Y, Yoshida M, Noji H, Kitakaze M, Komuro I, Asano Y, Takashima S
Proceedings of the National Academy of Sciences of the United States of America 2014 Jan 7;111(1):273-8

Prss37 is required for male fertility in the mouse.

Shen C, Kuang Y, Liu J, Feng J, Chen X, Wu W, Chi J, Tang L, Wang Y, Fei J, Wang Z
Biology of reproduction 2013 May;88(5):123

The liver connexin32 interactome is a novel plasma membrane-mitochondrial signaling nexus.

Fowler SL, Akins M, Zhou H, Figeys D, Bennett SA
Journal of proteome research 2013 Jun 7;12(6):2597-610

Protein sets define disease states and predict in vivo effects of drug treatment.

Meierhofer D, Weidner C, Hartmann L, Mayr JA, Han CT, Schroeder FC, Sauer S
Molecular & cellular proteomics : MCP 2013 Jul;12(7):1965-79

HtrA2 deficiency causes mitochondrial uncoupling through the F₁F₀-ATP synthase and consequent ATP depletion.

Plun-Favreau H, Burchell VS, Holmström KM, Yao Z, Deas E, Cain K, Fedele V, Moisoi N, Campanella M, Miguel Martins L, Wood NW, Gourine AV, Abramov AY
Cell death & disease 2012 Jun 28;3:e335

Relevance of the inner mitochondrial membrane enzyme F1F0-ATPase as an autoantigen in autoimmune liver disorders.

Preuss B, Berg C, Dengjel J, Stevanovic S, Klein R
Liver international : official journal of the International Association for the Study of the Liver 2012 Feb;32(2):249-57

Protein composition of immunoprecipitated synaptic ribbons.

Kantardzhieva A, Peppi M, Lane WS, Sewell WF
Journal of proteome research 2012 Feb 3;11(2):1163-74

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The ATP5A1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human ATP5A1. This antibody recognizes human,mouse,rat antigen. The ATP5A1 antibody has been validated for the following applications: ELISA, IHC, WB, IF analysis.
Appendix: 14676-1-AP.pdf

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using ATP5A1 antibody 14676-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human liver using 14676-1-AP(ATP5A1 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human liver using 14676-1-AP(ATP5A1 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue