43-9800
antibody from Invitrogen Antibodies
Targeting: ATP5F1A
ATP5A, ATP5A1, ATP5AL2, ATPM, hATP1, OMR, ORM
Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Flow cytometry [1]
- Other assay [2]
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Validation data
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- Product number
- 43-9800 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP5A1 Monoclonal Antibody (15H4C4)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- When performing IHC use heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Positive control: Human, bovine, mouse and rat heart mitochondria.
- Reactivity
- Human, Mouse, Rat, Bovine, Drosophila
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 15H4C4
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C
Submitted references mt tRFs, New Players in MELAS Disease.
mTORC2 protects the heart from high-fat diet-induced cardiomyopathy through mitochondrial fission in Drosophila.
Generation and Characterization of Germline-Specific Autophagy and Mitochondrial Reactive Oxygen Species Reporters in Drosophila.
Condensin II protein dysfunction impacts mitochondrial respiration and mitochondrial oxidative stress responses.
Mitochondrial Oxidative Phosphorylation defect in the Heart of Subjects with Coronary Artery Disease.
SNX14 mutations affect endoplasmic reticulum-associated neutral lipid metabolism in autosomal recessive spinocerebellar ataxia 20.
Regulation of autophagy, mitochondrial dynamics, and cellular bioenergetics by 4-hydroxynonenal in primary neurons.
Reducing Lissencephaly-1 levels augments mitochondrial transport and has a protective effect in adult Drosophila neurons.
Downstream effects of plectin mutations in epidermolysis bullosa simplex with muscular dystrophy.
Independent roles of methionine sulfoxide reductase A in mitochondrial ATP synthesis and as antioxidant in retinal pigment epithelial cells.
A chemical cross-linking method for the analysis of binding partners of heat shock protein-90 in intact cells.
Meseguer S, Rubio MP
Frontiers in physiology 2022;13:800171
Frontiers in physiology 2022;13:800171
mTORC2 protects the heart from high-fat diet-induced cardiomyopathy through mitochondrial fission in Drosophila.
Liu P, Chang K, Requejo G, Bai H
Frontiers in cell and developmental biology 2022;10:866210
Frontiers in cell and developmental biology 2022;10:866210
Generation and Characterization of Germline-Specific Autophagy and Mitochondrial Reactive Oxygen Species Reporters in Drosophila.
Nilangekar K, Murmu N, Sahu G, Shravage BV
Frontiers in cell and developmental biology 2019;7:47
Frontiers in cell and developmental biology 2019;7:47
Condensin II protein dysfunction impacts mitochondrial respiration and mitochondrial oxidative stress responses.
Deutschman E, Ward JR, Kumar A, Ray G, Welch N, Lemieux ME, Dasarathy S, Longworth MS
Journal of cell science 2019 Nov 20;132(22)
Journal of cell science 2019 Nov 20;132(22)
Mitochondrial Oxidative Phosphorylation defect in the Heart of Subjects with Coronary Artery Disease.
Ait-Aissa K, Blaszak SC, Beutner G, Tsaih SW, Morgan G, Santos JH, Flister MJ, Joyce DL, Camara AKS, Gutterman DD, Donato AJ, Porter GA Jr, Beyer AM
Scientific reports 2019 May 20;9(1):7623
Scientific reports 2019 May 20;9(1):7623
SNX14 mutations affect endoplasmic reticulum-associated neutral lipid metabolism in autosomal recessive spinocerebellar ataxia 20.
Bryant D, Liu Y, Datta S, Hariri H, Seda M, Anderson G, Peskett E, Demetriou C, Sousa S, Jenkins D, Clayton P, Bitner-Glindzicz M, Moore GE, Henne WM, Stanier P
Human molecular genetics 2018 Jun 1;27(11):1927-1940
Human molecular genetics 2018 Jun 1;27(11):1927-1940
Regulation of autophagy, mitochondrial dynamics, and cellular bioenergetics by 4-hydroxynonenal in primary neurons.
Dodson M, Wani WY, Redmann M, Benavides GA, Johnson MS, Ouyang X, Cofield SS, Mitra K, Darley-Usmar V, Zhang J
Autophagy 2017;13(11):1828-1840
Autophagy 2017;13(11):1828-1840
Reducing Lissencephaly-1 levels augments mitochondrial transport and has a protective effect in adult Drosophila neurons.
Vagnoni A, Hoffmann PC, Bullock SL
Journal of cell science 2016 Jan 1;129(1):178-90
Journal of cell science 2016 Jan 1;129(1):178-90
Downstream effects of plectin mutations in epidermolysis bullosa simplex with muscular dystrophy.
Winter L, Türk M, Harter PN, Mittelbronn M, Kornblum C, Norwood F, Jungbluth H, Thiel CT, Schlötzer-Schrehardt U, Schröder R
Acta neuropathologica communications 2016 Apr 27;4(1):44
Acta neuropathologica communications 2016 Apr 27;4(1):44
Independent roles of methionine sulfoxide reductase A in mitochondrial ATP synthesis and as antioxidant in retinal pigment epithelial cells.
Dun Y, Vargas J, Brot N, Finnemann SC
Free radical biology & medicine 2013 Dec;65:1340-1351
Free radical biology & medicine 2013 Dec;65:1340-1351
A chemical cross-linking method for the analysis of binding partners of heat shock protein-90 in intact cells.
Song S, Kole S, Bernier M
BioTechniques 2012 Apr;0(0):1-7
BioTechniques 2012 Apr;0(0):1-7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), NIH/3T3 (Lane 2), Hep G2 (Lane 3), COS-7 (Lane 4), MCF7 (Lane 5), MDA-MB-231 (Lane 6), RAW 264.7 (Lane 7), tissue extracts of Mouse Brain (Lane 8), Rat Brain (Lane 9), Mouse Colon (Lane 10) and Mouse lung (Lane 11). The blot was probed with Anti-ATP5A1 Monoclonal Antibody (15H4C4) (Product # 43-9800, 1 µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 55 kDa band corresponding to ATP5A1 was detected across the cell lines and tissues tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATP5A1 in isolated heart mitochondria extracts using a ATP5A1 Monoclonal antibody (Product # 43-9800) at a concentration of 1 µg/mL. Lane 1: Isolated mitochondria from human heart at 10 µg, Lane 2: Isolated mitochondria from bovine heart at 4 µg, Lane 3: Isolated mitochondria from rat heart at 10 µg. Lane 4: Isolated mitochondria from mouse heart at 10 µg, Lane 5: HepG2 (Human liver hepatocellular carcinoma cell line) lysate at 20 µg. Predicted band size: 53 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ATP5A1 was performed using HeLa cells. The cells were fixed with 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 for 5 minutes, and blocked with 1% BSA/10% normal goat serum/0.3 M glycine in 0.1% PBS-Tween for 1 hour. The cells were labeled with Tubulin Monoclonal Antibody and ATP5A1 Monoclonal Antibody (15H4C4) (Product # 43-9800) at 1 µg/mL, incubated at 4°C overnight and then incubated for 1 hour with Goat Anti-Mouse IgG (H+L) Secondary Antibody (Alexa Fluor® 647) preadsorbed at 0.5 µg/mL, Goat Anti-Rat IgG (H+L) Secondary Antibody (Alexa Fluor® 488) preadsorbed at 0.5 µg/mL and DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ATP5A1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ATP5A1 Monoclonal Antibody (15H4C4) (Product # 43-9800) at 2 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of ATP5A1 in HepG2 cells using a ATP5A1 Monoclonal Antibody (Product # 43-9800) at 1 µg/1x10^6 cells, shown in red. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) at 1:500 dilution. Isotype control, as seen in black, was a mouse IgG2b antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Partial localization of Dicer and Ago2 in the mitochondrial fraction of WT and MELAS cybrid cells. (A) Immunofluorescence microscopy analysis of endogenous Dicer and Ago2 subcellular localization in WT and MELAS cybrid cells. Nuclei were stained with DAPI (blue), and mitochondria were immunodetected with anti-ATP5A1 (OXPHOS Complex V subunit) or anti-CLPP (a serine protease located in the mitochondrial matrix). Scale bars, 10 mum. (B) Representative immunoblots of Dicer and Ago2 in subcellular fractions of WT and MELAS cybrid cells. Dicer and Ago2 levels were determined in nucleus and intact cells, cytosol and mitoplasts enriched fractions together with respective markers (Pol II (nucleus), Vinculin (cytoplasm), and SDHA (mitochondria)).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 4 Rictor controls HFD-induced mitochondrial localization of Drp1. (A) Immunostaining of mitochondrial localization of Drp1 protein in cardiomyocytes of ND- or HFD-treated control and rictor knockdown flies. Scale bar: 10 mum. (B) The quantification of Pearson correlation coefficient of the colocalization between Drp1 and mitochondrial marker (ATP5A1). Two-way ANOVA: Interaction between diet and genotype is highly significant, p < 0.0001. Tukey's multiple comparison test: *** p < 0.001, ns: not significant. N = 4~5 (4~5 hearts per genotype). (C) Immunostaining of mitochondria and F-actin of cardiomyocytes from ND- or HFD-treated control and Drp1 knockdown flies. Scale bar: 10 mum. (D) The proportion of the elongated mitochondria of the control and Drp1 knockdown flies under ND or HFD treatment. Two-way ANOVA: Interaction between diet and genotype is significant, p = 0.0098. Tukey's multiple comparison test: ** p < 0.01, ns: not significant. N = 4~6 (4~6 hearts per genotype). The control group re-uses the data from Figure 1F . (E) The proportion of the elongated mitochondria of the control, rictor knockdown, and rictor RNAi ; Drp1 OE flies under ND or HFD treatment. Two-way ANOVA: Interaction between diet and genotype is significant, p = 0.0072. Tukey's multiple comparison test: * p < 0.05, ns: not significant. N = 3~4 (3~4 hearts per genotype).