Explore
Validate
Learn
Western blot
ELISA
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [3]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-C498413 - Provider product page
- Provider
- LSBio
- Product name
- ATP5A1 / ATP Synthase Alpha Antibody LS-C498413
- Antibody type
- Polyclonal
- Description
- Protein G purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
No comments: Submit comment
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Positive WB detected in:Hela whole cell lysate,K562 whole cell lysate,MCF-7 whole cell lysate,PC3 whole cell lysate,Mouse heart tissue;All lanes: ATP5A1 antibody at 2.5ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 60,55,58 kDa;Observed band size: 60,58 kDa;
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in: K562 whole cell lysate, Mouse heart tissue All lanes: ATP5F1A antibody at 2.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 55, 58 kDa Observed band size: 60 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating ATP5A1 in Hela whole cell lysate;Lane 1: Rabbit monoclonal IgG(1ug)instead of ATP5F1A Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000);Lane 2: ATP5F1A Antibody(4ug)+ Hela whole cell lysate(500ug);Lane 3: Hela whole cell lysate (20ug);
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating ATP5A1 in Hela whole cell lysate;Lane 1: Rabbit monoclonal IgG(1ug)instead of ATP5F1A Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000);Lane 2: ATP5F1A Antibody(4ug)+ Hela whole cell lysate(500ug);Lane 3: Hela whole cell lysate (20ug);
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating ATP5A1 in Hela whole cell lysate;Lane 1: Rabbit monoclonal IgG(1ug)instead of ATP5F1A Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000);Lane 2: ATP5F1A Antibody(4ug)+ Hela whole cell lysate(500ug);Lane 3: Hela whole cell lysate (20ug);
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer using ATP5F1A Antibody at dilution of 1:100
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue using ATP5F1A Antibody at dilution of 1:100
