66037-1-Ig

antibody from Proteintech Group

Targeting: ATP5F1A ATP5A, ATP5A1, ATP5AL2, ATPM, hATP1, OMR, ORM

Provider product page for 66037-1-Ig

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [23]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 66037-1-Ig - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#66037-1-Ig, RRID:AB_11044196
Product name: ATP5A1 antibody
Antibody type: Monoclonal
Description: ATP5A1 antibody (Cat. #66037-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat, monkey and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat, Simian
Host: Mouse
Conjugate: Unconjugated
Isotype: IgG
Antibody clone number: 1B10H3
Vial size: 20ul, 150ul

ATP5F1A protein structure - 66037-1-Ig shown in red.

Submitted references Emodin Blocks mPTP Opening and Improves LPS-Induced HMEC-1 Cell Injury by Upregulation of ATP5A1.

Di T, He L, Shi Q, Chen L, Zhu L, Zhao S, Zhang C
Chemistry & biodiversity 2024 May;21(5):e202301916

Mulberry leaf flavonoids activate BAT and induce browning of WAT to improve type 2 diabetes via regulating the AMPK/SIRT1/PGC-1α signaling pathway.

Cheng L, Shi L, He C, Wang C, Lv Y, Li H, An Y, Duan Y, Dai H, Zhang H, Huang Y, Fu W, Sun W, Zhao B
Chinese journal of natural medicines 2023 Nov;21(11):812-829

Zhen Wu decoction represses renal fibrosis by invigorating tubular NRF2 and TFAM to fuel mitochondrial bioenergetics.

Zheng M, Hu Z, Wang Y, Wang C, Zhong C, Cui W, You J, Gao B, Sun X, La L
Phytomedicine : international journal of phytotherapy and phytopharmacology 2023 Jan;108:154495

The non-redundant functions of PIWI family proteins in gametogenesis in golden hamsters.

Lv X, Xiao W, Lai Y, Zhang Z, Zhang H, Qiu C, Hou L, Chen Q, Wang D, Gao Y, Song Y, Shui X, Chen Q, Qin R, Liang S, Zeng W, Shi A, Li J, Wu L
Nature communications 2023 Aug 29;14(1):5267

Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects.

Li D, Zhao Q, Xie L, Wang C, Tian Z, Tang H, Xia T, Wang A
The Science of the total environment 2023 Apr 15;869:161738

Inulin-type fructans change the gut microbiota and prevent the development of diabetic nephropathy.

Luo L, Luo J, Cai Y, Fu M, Li W, Shi L, Liu J, Dong R, Xu X, Tu L, Yang Y
Pharmacological research 2022 Sep;183:106367

CHCHD10 Modulates Thermogenesis of Adipocytes by Regulating Lipolysis.

Ding M, Ma YJ, Du RQ, Zhou WY, Dou X, Yang QQ, Tang Y, Qian SW, Liu Y, Pan DN, Tang QQ, Liu Y
Diabetes 2022 Sep 1;71(9):1862-1879

Berberine mitigates hepatic insulin resistance by enhancing mitochondrial architecture via the SIRT1/Opa1 signalling pathway.

Xu J, Zhang Y, Yu Z, Guan Y, Lv Y, Zhang M, Zhang M, Chen L, Lv X, Guan F
Acta biochimica et biophysica Sinica 2022 Oct 25;54(10):1464-1475

Geometric Constraints Regulate Energy Metabolism and Cellular Contractility in Vascular Smooth Muscle Cells by Coordinating Mitochondrial DNA Methylation.

Liu H, Liu Y, Wang H, Zhao Q, Zhang T, Xie SA, Liu Y, Tang Y, Peng Q, Pang W, Yao W, Zhou J
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022 Nov;9(32):e2203995

Rutin-activated adipose tissue thermogenesis is correlated with increased intestinal short-chain fatty acid levels.

Cheng L, Shi L, He C, Wang C, Lv Y, Li H, An Y, Dai H, Duan Y, Zhang H, Huang Y, Fu W, Meng Y, Zhao B
Phytotherapy research : PTR 2022 Jun;36(6):2495-2510

PDGF signaling inhibits mitophagy in glioblastoma stem cells through N(6)-methyladenosine.

Lv D, Gimple RC, Zhong C, Wu Q, Yang K, Prager BC, Godugu B, Qiu Z, Zhao L, Zhang G, Dixit D, Lee D, Shen JZ, Li X, Xie Q, Wang X, Agnihotri S, Rich JN
Developmental cell 2022 Jun 20;57(12):1466-1481.e6

Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.

Le Vasseur M, Friedman J, Jost M, Xu J, Yamada J, Kampmann M, Horlbeck MA, Salemi MR, Phinney BS, Weissman JS, Nunnari J
eLife 2021 May 26;10

AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.

Shi M, Huang XY, Ren XY, Wei XY, Ma Y, Lin ZZ, Liu DT, Song L, Zhao TJ, Li G, Yao L, Zhu M, Zhang C, Xie C, Wu Y, Wu HM, Fan LP, Ou J, Zhan YH, Lin SY, Lin SC
Nature cell biology 2021 Mar;23(3):268-277

Affinity-based protein profiling to reveal targets of puerarin involved in its protective effect on cardiomyocytes.

Huang S, Wang FJ, Lin H, Liu T, Zhao CX, Chen LG
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2021 Feb;134:111160

Assessment of SENP3-interacting proteins in hepatocytes treated with diethylnitrosamine by BioID assay.

Chen F, Yan H, Guo C, Zhu H, Yi J, Sun X, Yang J
Acta biochimica et biophysica Sinica 2021 Aug 31;53(9):1237-1246

Quantitative Mitochondrial Proteomics Reveals ANXA7 as a Crucial Factor in Mitophagy.

Meng K, Lu S, Yan X, Sun Y, Gao J, Wang Y, Yin X, Sun Z, He QY
Journal of proteome research 2020 Mar 6;19(3):1275-1284

A cross-talk between epithelium and endothelium mediates human alveolar-capillary injury during SARS-CoV-2 infection.

Wang P, Luo R, Zhang M, Wang Y, Song T, Tao T, Li Z, Jin L, Zheng H, Chen W, Zhao M, Zheng Y, Qin J
Cell death & disease 2020 Dec 8;11(12):1042

AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.

Ni Z, He J, Wu Y, Hu C, Dai X, Yan X, Li B, Li X, Xiong H, Li Y, Li S, Xu L, Li Y, Lian J, He F
Autophagy 2018;14(4):685-701

Region of Interest analysis using mass spectrometry imaging of mitochondrial and sarcomeric proteins in acute cardiac infarction tissue.

Yajima Y, Hiratsuka T, Kakimoto Y, Ogawa S, Shima K, Yamazaki Y, Yoshikawa K, Tamaki K, Tsuruyama T
Scientific reports 2018 May 10;8(1):7493

Succinate induces aberrant mitochondrial fission in cardiomyocytes through GPR91 signaling.

Lu YT, Li LZ, Yang YL, Yin X, Liu Q, Zhang L, Liu K, Liu B, Li J, Qi LW
Cell death & disease 2018 Jun 4;9(6):672

Succinate-induced neuronal mitochondrial fission and hexokinase II malfunction in ischemic stroke: Therapeutical effects of kaempferol.

Wu B, Luo H, Zhou X, Cheng CY, Lin L, Liu BL, Liu K, Li P, Yang H
Biochimica et biophysica acta. Molecular basis of disease 2017 Sep;1863(9):2307-2318

Ginkgolide K attenuates neuronal injury after ischemic stroke by inhibiting mitochondrial fission and GSK-3β-dependent increases in mitochondrial membrane permeability.

Zhou X, Wang HY, Wu B, Cheng CY, Xiao W, Wang ZZ, Yang YY, Li P, Yang H
Oncotarget 2017 Jul 4;8(27):44682-44693

Quantitative proteomic study of myocardial mitochondria in urea transporter B knockout mice.

Du Y, Meng Y, Zhu J, Kang L, Jia X, Guo L, Zhang L, Ye M, Hu L, Zhao X, Gu J, Yang B, Zou H
Proteomics 2014 Sep;14(17-18):2072-83

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Western blot analysis of ATP5A1 in various tissues and cell lines using Proteintech antibody 66037-1-Ig at the dilution of 1:1000.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using ATP5A1 antibody 66037-1-lg at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human heart using 66037-1-Ig(ATP5A1 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human heart using 66037-1-Ig(ATP5A1 antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue