RQ7217

antibody from NSJ Bioreagents

Targeting: ATP5F1A ATP5A, ATP5A1, ATP5AL2, ATPM, hATP1, OMR, ORM

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ7217

Provider

NSJ Bioreagents

Product name

ATP5A1 Antibody / ATP5F1A / ATP synthase subunit alpha

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the ATP5A1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat heart, 2) rat brain, 3) rat lung, 4) rat H9C2(2-1), 5) mouse heart, 6) mouse brain, 7) mouse lung and 8) mouse NIH 3T3 cell lysate with ATP5A1 antibody. Predicted molecular weight ~54-60 kDa (multiple isoforms).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) SK-OV-3, 2) MCF7, 3) HL60, 4) RT4, 5) Daudi, 6) ThP-1 and 7) HepG2 cell lysate with ATP5A1 antibody. Predicted molecular weight ~54-60 kDa (multiple isoforms).

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human placental tissue with ATP5A1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human colon cancer tissue with ATP5A1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human HepG2 cells with ATP5A1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human endometrial adenocarcinoma tissue with ATP5A1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human placental tissue with ATP5A1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human rectal cancer tissue with ATP5A1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human thyroid cancer tissue with ATP5A1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human U937 cells with ATP5A1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATP5A1 antibody.