LS-C375430

antibody from LSBio

Targeting: CD74 DHLAG

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C375430

Provider

LSBio

Product name

CD74 / CLIP Antibody (aa137-296) LS-C375430

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating CD74 in Raji whole cell lysate Lane 1: Rabbit control IgG instead of CD74 Antibody in Raji whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CD74 Antibody (8µg) + Raji whole cell lysate (500µg) Lane 3: Raji whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating CD74 in Raji whole cell lysate Lane 1: Rabbit control IgG instead of CD74 Antibody in Raji whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CD74 Antibody (8µg) + Raji whole cell lysate (500µg) Lane 3: Raji whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating CD74 in Raji whole cell lysate Lane 1: Rabbit control IgG instead of CD74 Antibody in Raji whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CD74 Antibody (8µg) + Raji whole cell lysate (500µg) Lane 3: Raji whole cell lysate (10µg)