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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00000972-M01A - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000972-M01A, RRID:AB_10550383
- Product name
- CD74 monoclonal antibody (M01A), clone 1D1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant CD74.
- Antigen sequence
MHRRRSRSCREDQKPVMDDQRDLISNNEQLPMLGR
RPGAPESKCSRGALYTGFSILVTLLLAGQATTAYF
LYQQQGRLDKLTVTSQNLQLENLRMKLPKPPKPVS
KMRMATPLLMQALPMGALPQGPMQNATKYGNMTED
HVMHLLQSHWNWRTRLLGWV- Isotype
- IgG
- Antibody clone number
- 1D1
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of CD74 expression in transfected 293T cell line by CD74 monoclonal antibody (M01A), clone 1D1.Lane 1: CD74 transfected lysate(18.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of CD74 transfected lysate using anti-CD74 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CD74 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
