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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- NBP2-59698 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CD74 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx 50kDa. No cross-reactivity against rat Cab Beta1b, Cav beta2a, and Cav betas3 in transfected cells.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: CD74 Antibody (6D9) [NBP2-59698] - Analysis of Human Lymphoblastoid cell line (Raji) showing detection of 33-35 kDa CD74 protein using Mouse Anti-CD74 Monoclonal Antibody, Clone 6D9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: Raji cell lysate. Load: 15 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 33-35 kDa.
