Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 12-0748-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD74 Monoclonal Antibody (5-329), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 5-329 monoclonal antibody recognizes human CD74, also known as the MHC Class II-associated invariant chain. A 33-43 kDa nonpolymorphic type II membrane protein, CD74 is highly expressed on B cells and subsets of activated T cells, Langerhans cells, dendritic cells, and epithelial cells. Monocytes and macrophages also express CD74, but at lower levels. CD74 acts as a chaperone of MHC Class II (HLA-DR) proteins, promoting their translocation from the ER to endocytic compartments during antigen presentation. Activation of NF\kappaB and ERK has been shown following CD74 interaction with CD44 and binding to the proinflammatory cytokine macrophage migration-inhibitory factor (MIF). CD74 also plays a role in the maturation of follicular B-cells and accumulation of marginal zone B-cells. CD74 has also been demonstrated to interact with CXCR2 and CXCR4. Applications Reported: This 5-329 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 5-329 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- 5-329
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease.
CD74 is a novel transcription regulator.
FOXP1 suppresses immune response signatures and MHC class II expression in activated B-cell-like diffuse large B-cell lymphomas.
High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells.
A functional heteromeric MIF receptor formed by CD74 and CXCR4.
Kinchen J, Chen HH, Parikh K, Antanaviciute A, Jagielowicz M, Fawkner-Corbett D, Ashley N, Cubitt L, Mellado-Gomez E, Attar M, Sharma E, Wills Q, Bowden R, Richter FC, Ahern D, Puri KD, Henault J, Gervais F, Koohy H, Simmons A
Cell 2018 Oct 4;175(2):372-386.e17
Cell 2018 Oct 4;175(2):372-386.e17
CD74 is a novel transcription regulator.
Gil-Yarom N, Radomir L, Sever L, Kramer MP, Lewinsky H, Bornstein C, Blecher-Gonen R, Barnett-Itzhaki Z, Mirkin V, Friedlander G, Shvidel L, Herishanu Y, Lolis EJ, Becker-Herman S, Amit I, Shachar I
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 17;114(3):562-567
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 17;114(3):562-567
FOXP1 suppresses immune response signatures and MHC class II expression in activated B-cell-like diffuse large B-cell lymphomas.
Brown PJ, Wong KK, Felce SL, Lyne L, Spearman H, Soilleux EJ, Pedersen LM, Møller MB, Green TM, Gascoyne DM, Banham AH
Leukemia 2016 Mar;30(3):605-16
Leukemia 2016 Mar;30(3):605-16
High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells.
Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T
Molecular & cellular proteomics : MCP 2016 Apr;15(4):1246-61
Molecular & cellular proteomics : MCP 2016 Apr;15(4):1246-61
A functional heteromeric MIF receptor formed by CD74 and CXCR4.
Schwartz V, Lue H, Kraemer S, Korbiel J, Krohn R, Ohl K, Bucala R, Weber C, Bernhagen J
FEBS letters 2009 Sep 3;583(17):2749-57
FEBS letters 2009 Sep 3;583(17):2749-57
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD74 PE (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 FOXP1 silencing increases HLA-DRA expression in ABC-DLBCL while FOXP1 transcripts are inversely correlated with antigen processing/presentation and with individual MHC II genes in primary DLBCL. ( a ) Knockdown of FOXP1 in OCI-Ly3 cells increased HLA-DRA and CD74 protein expression on the cell surface. Flow cytometry plots shown are representative of three independent experiments. ( b ) Gene Set Enrichment Analysis of primary DLBCL cases ( n =414; GSE10846) for gene sets associated with FOXP1 transcript expression; the 'antigen processing and presentation' signature was significantly enriched according to four independent FOXP1 probes ( P
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Colonic Mesenchymal Plasticity in IBD (A) t-SNE plot of UC colonic mesenchyme dataset. Single cells colored by cluster annotation. Descriptive cluster labels are shown. (B) Human healthy and UC cluster marker gene overlap correlation heatmap. (C) Selected enriched (FDR < 0.01) GO terms of UC S4 mesenchymal population marker genes. (D) (i) Flow cytometry analysis of CD74 and PDPN expression on colonic stromal cells from Ctrl (right) or UC (left) donors. (ii) Comparison of intracellular CCL19 and IL-33 levels in CD74 high PDPN high CD24 high cells (red) versus the corresponding CD74 low PDPN low subset (blue) in inflamed UC colonic tissue. (E) Flow cytometry analysis of FDCSP high and CD24 high colonic stromal cells from Ctrl (blue) or UC (red). (F) Single-molecule ISH staining of FDCSP in Ctrl or UC colonic tissue sections. (G) Flow cytometric analysis of SOX6 expression in Ctrl (blue) or UC (red) colonic stromal cells. See also Figures S1 and S3 and Tables S1 and S5 .
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CXCR4/CD74 receptor complex formation correlates with a functional interplay between CD74 and CXCR4. The CXCR4 inhibitor AMD3100 as well as anti-CXCR4 and anti-CD74 antibodies block MIF-mediated AKT activation in Jurkat T cells, whereas SDF-1alpha-mediated AKT activation is only reduced by anti-CXCR4 but not anti-CD74. (a) Flow cytometry histogram showing surface expression of CXCR4 in Jurkat T cells. Fluorescence intensity of anti-CXCR4-stained cells (green) is compared with that of cells stained with a non-specific FITC-IgG (grey). (b) Flow cytometry histogram showing surface expression of CD74 in Jurkat T cells. Fluorescence intensity of anti-CD74-stained cells (red) is compared with that of cells stained with a non-specific FITC-IgG (grey). (c) Jurkat cells pre-treated with AMD3100 (1 mug/ml, 30 min; +AMD3100) or with control buffer were incubated with rMIF at indicated concentrations for 10 min and AKT activation measured by Western blot using a phospho-Ser473-AKT antibody. Actin staining was performed for standardization of the blot. (d) Quantification of the blot according to (c) (phosphorylation ratio: AKT/actin; mean of n = 2). (e) As in c except that Jurkat cells were pre-treated with anti-CD74, anti-CXCR4 antibody or control IgG1 (10 mug/ml each, 30 min). MIF was added at a concentration of 100 ng/ml and for comparison cells were stimulated with 100 ng/ml SDF-1alpha. (f) Quantification of the blot according to (e) (phosphorylation ratio: AKT/actin; mean of n = 2).
- Conjugate
- Yellow dye