MA5-11966

antibody from Invitrogen Antibodies

Targeting: CD74 DHLAG

Antibody data

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Validation data

Product number: MA5-11966 - Provider product page
Provider: Invitrogen Antibodies
Product name: HLA-DR Monoclonal Antibody (LN3)
Antibody type: Monoclonal
Antigen: Other
Description: MA5-11966 targets Human MHC II (HLA-DR) in FACS, WB and IHC (F) applications and shows reactivity with Human samples. This antibody does not react with mouse tissue in Western blot applications.
Antibody clone number: LN3
Concentration: 0.2 mg/mL

CD74 protein structure - MA5-11966 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1 HLA-DR expression in human postmortem MSA brain tissue. Control (left) and MSA (middle and right) postmortem brain sections of the putamen (top) and substantia nigra (bottom) stained with HLA-DR (DAB, black staining). Quantification of the staining intensity revealed a significant increase of HLA-DR staining in the putamen and in the substantia nigra. Arrows denote ""resting"" (control, left) or ""activated"" (MSA, middle and right) microglia; arrowheads denote neuromelanin laden dopamine neurons in the substantia nigra. Representative images. All scale bars indicate 25 mum. Graphs display the mean +- SEM. * p < 0.05, unpaired nonparametric Mann Whitney Test was used to compare groups, n = 4 per group

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 (A) SR-SIM of CD4- and CD8-positive cells present in the perisaccular tissue. (B) Some cells express the toll-like receptor 4 (TLR4). (C) Sub-epithelial cell interaction near the external aperture of the vestibular aqueduct. IBA1 cells interact (framed area) with cells strongly expressing MHCII. Cell nuclei show different protein expression [from Kampfe-Nordstrom et al. () with permission]. (D) A sub-epithelial IBA1 cell contains a multi-vesicular body expressing MHCII.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 (A) Immunofluorescence of IBA1 and collagen IV in the lateral wall of the apical turn of the human cochlea. Many perivascular IBA1 cells are seen in the StV and few in the spiral ligament. (B) Confocal microscopy of the human StV. Framed area is magnified in (C) . (C) SR-SIM of framed area in B. Cell co-express IBA1 and MHCII. The cell membrane expresses MHCII as well as cytoplasmic vesicles [ (B,C) from Kampfe-Nordstrom et al. ( 12 )]. (D) Confocal microscopy of spiral ganglion with several surrounding IBA1 cells. SGC, satellite glial cell; Nu, type I cell nucleus; Col. IV, collagen IV; cap, capillary; E, endolymph.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1 Representative images of lesion types used in this study. Lesions are characterized by loss of PLP staining and amount of MHC-II+ cells. A large amount of of MHC-II+ cells can be observed in the demyelinated area ( a ) in active WMLs ( b ). Chronic-active demyelinated WMLs ( c ) feature a 'rim' of MHC-II cells ( d ) which is also visible in demyelinated active GMLs ( e, f ). Subpial demyelinated ( g ) GMLs hardly show MHC-II+ cells ( h ). Scalebar ( a-h ) = 200 mum. Dashed lines indicate the edge of the lesion

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 2 Representative images of MHC-II ( a, b, c ), Iba-1 ( d, e, f ), TMEM119 ( g, h, i ) and P2RY12 ( j, k, l ) immunoreactivity in normal appearing matter and in the demyelinated center of active WMLs and chronic-active WMLs and in pre-active lesions ( m, n, o, p ). Scalebars ( a-l and m-p ) = 50 mum

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 3 Representative images of MHC-II ( a, b, c ), Iba-1 ( d, e, f ), TMEM119 ( g, h, i ) and P2RY12 ( i, k,l ) immunoreactivity in NAGM, active subpial GMLs and non-active subpial GMLs. Arrows indicate rod-shaped microglia visible in subpial GMLs in Iba-1+ cells ( f ), TMEM119 + cells ( i ) and P2RY12+ cells ( l ). Scalebar = 50 mum

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 5 Representative images of immunoreactivity for MHC-II ( a, b, c ), Iba-1 ( d, e, f ), TMEM119 ( g, h, i ) and P2RY11 ( j, k, l ) along the rim of various lesion types. Scalebar ( a-l ) = 50 mum