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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- NBP1-33109 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33109, RRID:AB_10004032
- Product name
- Rabbit Polyclonal CD74 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Expression of macrophage migration inhibitory factor and CD74 in the inner ear and middle ear in lipopolysaccharide-induced otitis media.
A conserved WW domain-like motif regulates invariant chain-dependent cell-surface transport of the NKG2D ligand ULBP2.
The NKG2D ligand ULBP2 is specifically regulated through an invariant chain-dependent endosomal pathway.
Ishihara H, Kariya S, Okano M, Zhao P, Maeda Y, Nishizaki K
Acta oto-laryngologica 2016 Oct;136(10):1011-6
Acta oto-laryngologica 2016 Oct;136(10):1011-6
A conserved WW domain-like motif regulates invariant chain-dependent cell-surface transport of the NKG2D ligand ULBP2.
Uhlenbrock F, van Andel E, Andresen L, Skov S
Molecular immunology 2015 Aug;66(2):418-27
Molecular immunology 2015 Aug;66(2):418-27
The NKG2D ligand ULBP2 is specifically regulated through an invariant chain-dependent endosomal pathway.
Uhlenbrock F, Hagemann-Jensen M, Kehlet S, Andresen L, Pastorekova S, Skov S
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 15;193(4):1654-65
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 15;193(4):1654-65
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD74 Antibody [NBP1-33109] - A. 30 ug Rat2 whole cell lysate/extract 12 % SDS-PAGE CD74 antibody [N1N2], N-term dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD74 Antibody [NBP1-33109] - A. 30 ug GL261 whole cell lysate/extract 12 % SDS-PAGE CD74 antibody [N1N2], N-term dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD74 Antibody [NBP1-33109] - Whole cell extracts (30ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CD74 antibody at a dilution of 1:5000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD74 Antibody [NBP1-33109] - Human lung carcinoma. CD74 antibody [N1N2], N-term diluted at 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min
