Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- NB100-1985 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1985, RRID:AB_10001511
- Product name
- Mouse Monoclonal CD74 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This detects an ~33-35 kDa protein doublet, corresponding to the apparent molecular mass of the p33 and p35 forms of human CD74.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Antigen sequence
The epitope is in a region of the c
ytoplasmic tail of human CD74 which
is common to all isoforms.- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Cell Surface CD74-MIF Interactions Drive Melanoma Survival in Response to Interferon-γ.
Gain in brain immunity in the oldest-old differentiates cognitively normal from demented individuals.
Assembly and transport properties of invariant chain trimers and HLA-DR-invariant chain complexes.
Formation of a nine-subunit complex by HLA class II glycoproteins and the invariant chain.
Tanese K, Hashimoto Y, Berkova Z, Wang Y, Samaniego F, Lee JE, Ekmekcioglu S, Grimm EA
The Journal of investigative dermatology 2015 Nov;135(11):2775-2784
The Journal of investigative dermatology 2015 Nov;135(11):2775-2784
Gain in brain immunity in the oldest-old differentiates cognitively normal from demented individuals.
Katsel P, Tan W, Haroutunian V
PloS one 2009 Oct 29;4(10):e7642
PloS one 2009 Oct 29;4(10):e7642
Assembly and transport properties of invariant chain trimers and HLA-DR-invariant chain complexes.
Lamb CA, Cresswell P
Journal of immunology (Baltimore, Md. : 1950) 1992 Jun 1;148(11):3478-82
Journal of immunology (Baltimore, Md. : 1950) 1992 Jun 1;148(11):3478-82
Formation of a nine-subunit complex by HLA class II glycoproteins and the invariant chain.
Roche PA, Marks MS, Cresswell P
Nature 1991 Dec 5;354(6352):392-4
Nature 1991 Dec 5;354(6352):392-4
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD74 Antibody (PIN.1) [NB100-1985] - Western blot of CD74 from IP N87 lysates mixed with macrophage migration inhibitory factor (MIF).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD74 Antibody (PIN.1) [NB100-1985] - Ramos whole cell protein was separated on a 4-12% gel by SDS-PAGE and protein transferred to PVDF. The membrane was probed with anti-CD74 at 2 ug/ml and detected with and anti-mouse HRP secondary antibody using chemiluminescence. The arrow head shows the detection of CD74.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [NB100-1985] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human spleen using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [NB100-1985] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human renal cell carcinoma using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [NB100-1985] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human pulmonary squamous cell carcinoma using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD74 Antibody (PIN.1) [NB100-1985] - CD74 in human PBMCs (gated on all monocytes). Orange represents isotype control and blue represents anti-CD74 antibody staining.