20538-1-AP

antibody from Invitrogen Antibodies

Targeting: EFS CASS3, EFS1, EFS2, HEFS, SIN

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

20538-1-AP

Provider

Invitrogen Antibodies

Product name

EFS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: TPKPPAELE PDLEWEGGRE PGPPIYAAPS NLKRASALLN LYEAPEELLA DGEGGGTDEG IYDVPLLGPE APPSPEPPGA LASHDQDTLA QLLARSPPPP HRPRLPSAES LSRRPLPALP VPEAPSPSPV PSPAPGRKGS IQDRPLPPPP PRLPGYGGPK VEGDPEGREM EDDPAGHHNE YEGIPMAEEY DYVHLKGMDK AQGSRPPDQA CTGDPELPER GMPAPQEALS PGEPLVVSTG DLQLLYFYAG QCQSHYSALQ AAVAALMSST QANQPPRLFV PHSKRVVVAA HRLVFVGDTL GR (100-400 aa encoded by BC034246)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human placenta tissue were subjected to SDS PAGE followed by western blot with 20538-1-AP (EFS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 20538-1-AP (EFS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20538-1-AP (EFS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using EFS antibody 20538-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human spleen tissue slide using 20538-1-AP (EFS antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human spleen tissue slide using 20538-1-AP (EFS antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-EFS (IP:20538-1-AP, 4ug; Detection:20538-1-AP 1:300) with mouse thymus tissue lysate 3200ug.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-EFS (IP:20538-1-AP, 4 ug; Detection:20538-1-AP 1:300) with mouse thymus tissue lysate 2800 ug.