IC1258A

antibody from Novus Biologicals

Targeting: IL25 IL-17E, IL-25, IL17E

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC1258A

Provider

Novus Biologicals

Product name

Mouse Monoclonal IL-17E/IL-25 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human IL-17E in direct ELISAs and Western blots. Shows 100% cross-reactivity with recombinant mouse IL-17E and no cross-reactivity with recombinant human (rh) IL-17, rhIL-17B, rhIL-17C, or rhIL-17F.

Reactivity

Human

Host

Mouse

Conjugate

Red dye

Isotype

IgG

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.

References

Submitted references

Interleukin-25: a cytokine linking eosinophils and adaptive immunity in Churg-Strauss syndrome.

Terrier B, Bièche I, Maisonobe T, Laurendeau I, Rosenzwajg M, Kahn JE, Diemert MC, Musset L, Vidaud M, Sène D, Costedoat-Chalumeau N, Le Thi-Huong D, Amoura Z, Klatzmann D, Cacoub P, Saadoun D
Blood 2010 Nov 25;116(22):4523-31

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of IL-17E/IL-25 in PC-3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human IL-17E/IL-25 APC-conjugated Monoclonal Antibody (Catalog # IC1258A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.