Explore
Validate
Learn
ELISA
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB13991-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-17E/IL-25 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-17E/IL-25 in ELISAs. In ELISAs, no cross-reactivity with recombinant human IL-17E, recombinant mouse (rm) IL-17, rmIL-17B, C, D, or F is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
NP_542767- Isotype
- IgG
- Antibody clone number
- 207702
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-17E/IL-25 in Tramp-C1 Mouse Cell Line by Flow Cytometry. Tramp-C1 mouse prostate cancer cell line was stained with Mouse IL-17E/IL-25 Monoclonal Antibody (Catalog # MAB13991, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
